Literature DB >> 34595305

Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry.

Antonio Lagares1, Claudio Valverde1.   

Abstract

We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Bacteria; Flow cytometry; Nile red; PHB; Polyhydroxybutyrate

Year:  2017        PMID： 34595305      PMCID： PMC8438502          DOI： 10.21769/BioProtoc.2638

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

1.  Assay of poly-beta-hydroxybutyric acid.

Authors:  J H LAW; R A SLEPECKY
Journal:  J Bacteriol       Date:  1961-07       Impact factor: 3.490

2.  Staining and quantification of poly-3-hydroxybutyrate in Saccharomyces cerevisiae and Cupriavidus necator cell populations using automated flow cytometry.

Authors:  James Kacmar; Ross Carlson; Steven J Balogh; Friedrich Srienc
Journal:  Cytometry A       Date:  2006-01       Impact factor: 4.355

3.  Quantification of bacterial polyhydroxyalkanoic acids by Nile red staining.

Authors:  V Gorenflo; A Steinbüchel; S Marose; M Rieseberg; T Scheper
Journal:  Appl Microbiol Biotechnol       Date:  1999-06       Impact factor: 4.813

4.  Regulation of Polyhydroxybutyrate Accumulation in Sinorhizobium meliloti by the Trans-Encoded Small RNA MmgR.

Authors:  Antonio Lagares; Germán Ceizel Borella; Uwe Linne; Anke Becker; Claudio Valverde
Journal:  J Bacteriol       Date:  2017-03-28       Impact factor: 3.490

5.  High-throughput screen for poly-3-hydroxybutyrate in Escherichia coli and Synechocystis sp. strain PCC6803.

Authors:  Keith E Tyo; Hang Zhou; Gregory N Stephanopoulos
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

Review 6.  New insights in the formation of polyhydroxyalkanoate granules (carbonosomes) and novel functions of poly(3-hydroxybutyrate).

Authors:  Dieter Jendrossek; Daniel Pfeiffer
Journal:  Environ Microbiol       Date:  2014-01-21       Impact factor: 5.491

7.  Poly-3-hydroxybutyrate (PHB) supports survival and reproduction in starving rhizobia.

Authors:  William C Ratcliff; Supriya V Kadam; Robert Ford Denison
Journal:  FEMS Microbiol Ecol       Date:  2008-07-08       Impact factor: 4.194

8.  Spectrofluorometric studies of the lipid probe, nile red.

Authors:  P Greenspan; S D Fowler
Journal:  J Lipid Res       Date:  1985-07       Impact factor: 5.922

9.  A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry.

Authors:  L P S Alves; A T Almeida; L M Cruz; F O Pedrosa; E M de Souza; L S Chubatsu; M Müller-Santos; G Valdameri
Journal:  Braz J Med Biol Res       Date:  2017-01-16       Impact factor: 2.590
  9 in total

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