Literature DB >> 34595275

Monitoring the Targeting of Cathepsin D to the Lysosome by Metabolic Labeling and Pulse-chase Analysis.

Lucas A Tavares1, Luis L P daSilva1.   

Abstract

Mannose 6-phosphate receptors function can be studied in living cells by investigating alterations in processing and secretion of their ligand Cathepsin D. The assay described here is well established in the literature and comprises the metabolic labeling of newly synthesized proteins with [35S] methionine-cysteine in HeLa cells to monitor Cathepsin D processing through secretory pathway and secretion using immunoprecipitation, SDS-PAGE and fluorography.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Acid hydrolases; Cathepsin D; Lysosomes; MPR; Mannose 6-phosphate receptors; Metabolic labeling; Pulse-chase; Secretory pathway

Year:  2017        PMID： 34595275      PMCID： PMC8438373          DOI： 10.21769/BioProtoc.2598

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

Review 1.  Mannose 6-phosphate receptors: new twists in the tale.

Authors:  Pradipta Ghosh; Nancy M Dahms; Stuart Kornfeld
Journal:  Nat Rev Mol Cell Biol       Date:  2003-03       Impact factor: 94.444

2.  The immunocytochemical demonstration of cathepsin D.

Authors:  A R Poole; J T Dingle; A J Barrett
Journal:  J Histochem Cytochem       Date:  1972-04       Impact factor: 2.479

3.  CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the γ1 and γ2 subunits of the AP-1 complex in protein trafficking.

Authors:  Lucas A Tavares; Eulália M L da Silva; Mara E da Silva-Januário; Yunan C Januário; Julianne V de Cavalho; Érika S Czernisz; Gonzalo A Mardones; Luis L P daSilva
Journal:  J Cell Sci       Date:  2016-12-01       Impact factor: 5.285

Review 4.  Human cathepsin D.

Authors:  Alina Minarowska; Marek Gacko; Alicja Karwowska; Łukasz Minarowski
Journal:  Folia Histochem Cytobiol       Date:  2008       Impact factor: 1.698

5.  Spatial and functional relationship of GGAs and AP-1 in Drosophila and HeLa cells.

Authors:  Jennifer Hirst; Daniela A Sahlender; Maja Choma; Rita Sinka; Michael E Harbour; Michael Parkinson; Margaret S Robinson
Journal:  Traffic       Date:  2009-11       Impact factor: 6.215

6.  Canonical interaction of cyclin G associated kinase with adaptor protein 1 regulates lysosomal enzyme sorting.

Authors:  Satoshi Kametaka; Kengo Moriyama; Patricia V Burgos; Evan Eisenberg; Lois E Greene; Rafael Mattera; Juan S Bonifacino
Journal:  Mol Biol Cell       Date:  2007-05-30       Impact factor: 4.138

Review 7.  Cathepsin D--many functions of one aspartic protease.

Authors:  Petr Benes; Vaclav Vetvicka; Martin Fusek
Journal:  Crit Rev Oncol Hematol       Date:  2008-04-08       Impact factor: 6.312

8.  Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococcus pneumoniae.

Authors:  Martin A Bewley; Trong K Pham; Helen M Marriott; Josselin Noirel; Hseuh-Ping Chu; Saw Y Ow; Alexey G Ryazanov; Robert C Read; Moira K B Whyte; Benny Chain; Phillip C Wright; David H Dockrell
Journal:  Mol Cell Proteomics       Date:  2011-04-07       Impact factor: 5.911
  8 in total

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