Literature DB >> 34595269

Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat.

Jinkun Wen1, Dandan Tan1, Lixia Li1, Jiasong Guo1,2,3.   

Abstract

Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal Sprague-Dawley (SD) rats. In the present protocol, dissociated SCs are isolated from the spinal nerves of neonatal rats and purified by the treatment of cytosine arabinoside (AraC).
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Isolation; Purification; Rat; Schwann cell; Spinal nerve

Year:  2017        PMID： 34595269      PMCID： PMC8438390          DOI： 10.21769/BioProtoc.2588

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  11 in total

1.  Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve.

Authors:  Maurice Vroemen; Norbert Weidner
Journal:  J Neurosci Methods       Date:  2003-04-15       Impact factor: 2.390

2.  A new method of selecting Schwann cells from adult mouse sciatic nerve.

Authors:  Michael E Pannunzio; I-ming Jou; Andrew Long; Tyler C Wind; Gina Beck; Gary Balian
Journal:  J Neurosci Methods       Date:  2005-06-20       Impact factor: 2.390

3.  Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment.

Authors:  Yu-Qing Jin; Wei Liu; Tan-Hui Hong; Yilin Cao
Journal:  J Neurosci Methods       Date:  2008-01-16       Impact factor: 2.390

4.  Separation of functional Schwann cells and neurons from normal peripheral nerve tissue.

Authors:  P M Wood
Journal:  Brain Res       Date:  1976-10-22       Impact factor: 3.252

5.  Isolation and culture of Schwann cells.

Authors:  Yanmei Tao
Journal:  Methods Mol Biol       Date:  2013

6.  A simple method for the Schwann cell preparation from newborn rat sciatic nerves.

Authors:  Y Oda; Y Okada; S Katsuda; K Ikeda; I Nakanishi
Journal:  J Neurosci Methods       Date:  1989-06       Impact factor: 2.390

7.  Purification of rat Schwann cells from cultures of peripheral nerve: an immunoselective method using surfaces coated with anti-immunoglobulin antibodies.

Authors:  J G Assouline; E P Bosch; R Lim
Journal:  Brain Res       Date:  1983-10-31       Impact factor: 3.252

8.  Human and rat adult Schwann cell cultures: fast and efficient enrichment and highly effective non-viral transfection protocol.

Authors:  Kirsten Haastert; Christina Mauritz; Sukhada Chaturvedi; Claudia Grothe
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

9.  Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve.

Authors:  J P Brockes; K L Fields; M C Raff
Journal:  Brain Res       Date:  1979-04-06       Impact factor: 3.252

10.  Lentivirus-Mediated RNA Interference Targeting RhoA Slacks the Migration, Proliferation, and Myelin Formation of Schwann Cells.

Authors:  Jinkun Wen; Changhui Qian; Mengjie Pan; Xianghai Wang; Yuanyuan Li; Yanmeng Lu; Zhitao Zhou; Qing Yan; Lixia Li; Zhongying Liu; Wutian Wu; Jiasong Guo
Journal:  Mol Neurobiol       Date:  2016-01-28       Impact factor: 5.590
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