Literature DB >> 34595249

Quantification of Densities of Bacterial Endosymbionts of Insects by Real-time PCR.

Daisuke Kageyama1.   

Abstract

Increased attention has been paid to the endosymbiotic bacteria of insects. Because most insect endosymbionts are uncultivable, quantitative PCR (qPCR) is a practical and convenient method to quantify endosymbiont titers. Here we report a protocol for real-time qPCR based on SYBR Green I fluorescence as well as some tips to prevent possible pitfalls.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Bacteria; Endosymbionts; Insects; Quantification; SYBR Green; qPCR

Year:  2017        PMID： 34595249      PMCID： PMC8438360          DOI： 10.21769/BioProtoc.2566

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  15 in total

1.  Real-time PCR for quantification of the bacterial endosymbionts (Wolbachia) of filarial nematodes.

Authors:  L Simoncini; M Casiraghi; C Bazzocchi; L Sacchi; C Bandi; C Genchi
Journal:  Parassitologia       Date:  2001-12

2.  Novel strain of Spiroplasma found in flower bugs of the genus Orius (Hemiptera: Anthocoridae): transovarial transmission, coexistence with Wolbachia and varied population density.

Authors:  Masaya Watanabe; Fumiko Yukuhiro; Taro Maeda; Kazuki Miura; Daisuke Kageyama
Journal:  Microb Ecol       Date:  2013-12-17       Impact factor: 4.552

3.  Asymmetrical interactions between Wolbachia and Spiroplasma endosymbionts coexisting in the same insect host.

Authors:  Shunsuke Goto; Hisashi Anbutsu; Takema Fukatsu
Journal:  Appl Environ Microbiol       Date:  2006-07       Impact factor: 4.792

Review 4.  Genetics, physiology, and evolutionary relationships of the genus Buchnera: intracellular symbionts of aphids.

Authors:  P Baumann; L Baumann; C Y Lai; D Rouhbakhsh; N A Moran; M A Clark
Journal:  Annu Rev Microbiol       Date:  1995       Impact factor: 15.500

5.  Widespread lateral gene transfer from intracellular bacteria to multicellular eukaryotes.

Authors:  Julie C Dunning Hotopp; Michael E Clark; Deodoro C S G Oliveira; Jeremy M Foster; Peter Fischer; Mónica C Muñoz Torres; Jonathan D Giebel; Nikhil Kumar; Nadeeza Ishmael; Shiliang Wang; Jessica Ingram; Rahul V Nene; Jessica Shepard; Jeffrey Tomkins; Stephen Richards; David J Spiro; Elodie Ghedin; Barton E Slatko; Hervé Tettelin; John H Werren
Journal:  Science       Date:  2007-08-30       Impact factor: 47.728

6.  Wolbachia and virus protection in insects.

Authors:  Lauren M Hedges; Jeremy C Brownlie; Scott L O'Neill; Karyn N Johnson
Journal:  Science       Date:  2008-10-31       Impact factor: 47.728

7.  Facultative bacterial symbionts in aphids confer resistance to parasitic wasps.

Authors:  Kerry M Oliver; Jacob A Russell; Nancy A Moran; Martha S Hunter
Journal:  Proc Natl Acad Sci U S A       Date:  2003-01-31       Impact factor: 11.205

8.  Naturally occurring single and double infection with Wolbachia strains in the butterfly Eurema hecabe: transmission efficiencies and population density dynamics of each Wolbachia strain.

Authors:  Satoko Narita; Masashi Nomura; Daisuke Kageyama
Journal:  FEMS Microbiol Ecol       Date:  2007-05-16       Impact factor: 4.194

Review 9.  Insect Sex Determination Manipulated by Their Endosymbionts: Incidences, Mechanisms and Implications.

Authors:  Daisuke Kageyama; Satoko Narita; Masaya Watanabe
Journal:  Insects       Date:  2012-02-10       Impact factor: 2.769

10.  A Nightmare for Males? A Maternally Transmitted Male-Killing Bacterium and Strong Female Bias in a Green Lacewing Population.

Authors:  Masayuki Hayashi; Masaya Watanabe; Fumiko Yukuhiro; Masashi Nomura; Daisuke Kageyama
Journal:  PLoS One       Date:  2016-06-15       Impact factor: 3.240
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