Sustained and enhanced anaerobic removal of COD and nitrogen in a zeolite amended glycogen accumulating organism dominated biofilm process.

Activated sludge, the most widely used biological wastewater treatment process is known to be expensive to operate, largely due to energy expense for oxygen transfer into the bulk wastewater solution. The alternative of using passive aeration facilitates oxygen supply directly from the air resulting in aeration energy savings. The current study demonstrated sustained and improved removal of chemical oxygen demand (COD) and nitrogen in a zeolite modified glycogen accumulating organisms (GAOs) dominated biofilm reactor, which achieved anaerobic removal of COD and ammonium by the activity of GAOs and zeolite, respectively. Draining of the batch-operated reactor enabled the biofilm to directly uptake oxygen from air (passive aeration) to carry out simultaneous nitrification and denitrification due to the activity of GAO (Candidatus competibacter) and nitrifying bacteria (Nitrosomonas and Nitrospira). Under stable long-term (4-months) operation, the process achieved COD and nitrogen removal at rates of 1354 and 79.1 g m-3 d-1, respectively. The biofilm process demonstrated >90% nitrogen removal efficiency in multi-cycle (4/8 cycles) strategy with a short treatment time of 8 h. Due to the passive aeration scheme, the energy consumption of the proposed wastewater treatment process is calculated to be about 13-times less than that of traditional activated sludge process. Therefore, the Passive Aeration Simultaneous Nitrification and Denitrification (PASND) biofilm process is a promising low-energy treatment step for efficient removal of COD and nitrogen from wastewater.