Literature DB >> 3459169

nu 1, a Mr 43,000 component of postsynaptic membranes, is a protein kinase.

A S Gordon, D Milfay.   

Abstract

Acetylcholine receptor-enriched membranes from the electric organ of Torpedo californica show a major band at Mr 43,000 on NaDodSO4/polyacrylamide gels. This band is composed of three polypeptides: nu 1, nu 2, and nu 3. Polypeptide nu 1 has been found to be localized exclusively at the innervated face of the electrocyte and at the neuromuscular junction in rat muscle. We show here that monoclonal antibody to nu 1 precipitates a radioactive Mr 43,000 polypeptide from detergent-solubilized extracts of Torpedo membranes covalently labeled with periodate-oxidized [alpha-32P]ATP. The monoclonal antibody also precipitates protein kinase activity from neutralized pH 11 extracts of the acetylcholine receptor-rich membranes. These data suggest that nu 1 is a postsynaptic membrane protein kinase.

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Year:  1986        PMID： 3459169      PMCID： PMC323693          DOI： 10.1073/pnas.83.12.4172

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

2.  Phosphorylation of acetylcholine receptor by endogenous membrane protein kinase in receptor-enriched membranes of Torpedo californica.

Authors:  A S Gordon; C G Davis; D Milfay; I Diamond
Journal:  Nature       Date:  1977-06-09       Impact factor: 49.962

3.  Phosphorylation of membrane proteins at a cholinergic synapse.

Authors:  A S Gordon; C G Davis; I Diamond
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  The subsynaptic 43-kDa protein is concentrated at developing nerve-muscle synapses in vitro.

Authors:  S J Burden
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

6.  In vitro phosphorylation of the acetylcholine receptor.

Authors:  V I Teichberg; A Sobel; J P Changeux
Journal:  Nature       Date:  1977-06-09       Impact factor: 49.962

7.  Immunofluorescence localization at the mammalian neuromuscular junction of the Mr 43,000 protein of Torpedo postsynaptic membranes.

Authors:  S C Froehner; V Gulbrandsen; C Hyman; A Y Jeng; R R Neubig; J B Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

8.  Structural heterogeneity and subcellular distribution of nicotinic synapse-associated proteins.

Authors:  R Gysin; M Wirth; S D Flanagan
Journal:  J Biol Chem       Date:  1981-11-25       Impact factor: 5.157

9.  Purification of Torpedo californica post-synaptic membranes and fractionation of their constituent proteins.

Authors:  J Elliott; S G Blanchard; W Wu; J Miller; C D Strader; P Hartig; H P Moore; J Racs; M A Raftery
Journal:  Biochem J       Date:  1980-03-01       Impact factor: 3.857

10.  Acetylcholine and local anesthetic binding to Torpedo nicotinic postsynaptic membranes after removal of nonreceptor peptides.

Authors:  R R Neubig; E K Krodel; N D Boyd; J B Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205
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  4 in total

Review 1.  Regulation of nicotinic acetylcholine receptors by protein phosphorylation.

Authors:  K Miles; R L Huganir
Journal:  Mol Neurobiol       Date:  1988       Impact factor: 5.590

2.  cDNAs for the postsynaptic 43-kDa protein of Torpedo electric organ encode two proteins with different carboxyl termini.

Authors:  D E Frail; J Mudd; V Shah; C Carr; J B Cohen; J P Merlie
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

Review 3.  The submembrane machinery for nicotinic acetylcholine receptor clustering.

Authors:  S C Froehner
Journal:  J Cell Biol       Date:  1991-07       Impact factor: 10.539

4.  The mammalian 43-kD acetylcholine receptor-associated protein (RAPsyn) is expressed in some nonmuscle cells.

Authors:  L S Musil; D E Frail; J P Merlie
Journal:  J Cell Biol       Date:  1989-05       Impact factor: 10.539
  4 in total

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