Adem Akçakaya1, Nurcan Ünver2, Tuğba Aydoğan Kiriş3, Mehmet Güzel4, Fatma Betül Akçakaya5, Bedia Çakmakoğlu6, Mustafa Hasbahçeci7,8. 1. Department of General Surgery, Bezmialem Vakif University, Faculty of Medicine, İstanbul, Turkey. 2. Clinic of Pathology, Yedikule Chest Diseases and Thoracic Surgery Training and Research Hospital, İstanbul, Turkey. 3. Graduate School of Science, İstanbul University, İstanbul, Turkey. 4. Clinic of General Surgery, Patnos State Hospital, Ağrı, Turkey. 5. Bezmialem Vakif University, Faculty of Medicine, İstanbul, Turkey. 6. Department of Molecular Medicine, Istanbul University Aziz Sancar Institute of Experimental Medicine, İstanbul, Turkey. 7. Department of General Surgery, Istanbul Istinye University, Faculty of Medicine, İstanbul, Turkey. 8. A.D.E.M., Center for Academic Support and Education, Istanbul, Turkey.
Abstract
OBJECTIVES: The loss of function of the E-cadherin (CDH1) gene with -160 C→A and -347 G→GA polymorphisms is regarded as a critical step for gastric cancer. It was aimed to investigate possible association of these polymorphisms and immunoexpression of E-cadherin with gastric cancer. MATERIAL AND METHODS: Gastric adenocarcinoma patients and individuals with benign gastric pathologies were included in this case-control study. Demographic data and pathological findings were recorded. Immunohistochemical staining of E-cadherin expression and analysis of -160 C→A and -347 G→GA polymorphisms were done. Differences between allele frequencies of -160 C→A and -347 G→GA polymorphisms and expression of E-cadherin were the primary outcomes. RESULTS: There were 78 gastric cancer patients (Group A) and 113 individuals with benign gastric pathologies (Group B). The number of male patients and mean age were higher in Group A (p <0.001). -160 C→A and 347 G→GA polymorphisms and their allelic distributions showed no difference between the groups (p> 0.05 for all). There was a significant association between -160 C→A polymorphism and grade of E-cadherin expression (p= 0.013). There were no significant differences between survival rates with -160 C→A, 347 G→GA and intensity of E-cadherin expression (p> 0.05 for all). There was no significant association between -160 C→A and -347 G→GA polymorphisms and gastric cancer. CONCLUSION: There was no impact of E-cadherin expression on tumoral features and survival in gastric cancer. -160 C→A polymorphism may influence the expression of E-cadherin in gastric cancer.
OBJECTIVES: The loss of function of the E-cadherin (CDH1) gene with -160 C→A and -347 G→GA polymorphisms is regarded as a critical step for gastric cancer. It was aimed to investigate possible association of these polymorphisms and immunoexpression of E-cadherin with gastric cancer. MATERIAL AND METHODS: Gastric adenocarcinoma patients and individuals with benign gastric pathologies were included in this case-control study. Demographic data and pathological findings were recorded. Immunohistochemical staining of E-cadherin expression and analysis of -160 C→A and -347 G→GA polymorphisms were done. Differences between allele frequencies of -160 C→A and -347 G→GA polymorphisms and expression of E-cadherin were the primary outcomes. RESULTS: There were 78 gastric cancer patients (Group A) and 113 individuals with benign gastric pathologies (Group B). The number of male patients and mean age were higher in Group A (p <0.001). -160 C→A and 347 G→GA polymorphisms and their allelic distributions showed no difference between the groups (p> 0.05 for all). There was a significant association between -160 C→A polymorphism and grade of E-cadherin expression (p= 0.013). There were no significant differences between survival rates with -160 C→A, 347 G→GA and intensity of E-cadherin expression (p> 0.05 for all). There was no significant association between -160 C→A and -347 G→GA polymorphisms and gastric cancer. CONCLUSION: There was no impact of E-cadherin expression on tumoral features and survival in gastric cancer. -160 C→A polymorphism may influence the expression of E-cadherin in gastric cancer.
Authors: Heriberto Medina-Franco; Antonio Ramos-De la Medina; Gloria Vizcaino; Jose Luis Medina-Franco Journal: Ann Surg Oncol Date: 2007-06-05 Impact factor: 5.344