Literature DB >> 34585

A quantitative cytochemical method for the demonstration of delta5,3beta-hydroxysteroid dehydrogenase activity in unfixed tissue sections of rat ovary.

W R Robertson.   

Abstract

A method for the quantitative measurement of delta5,3beta-hydroxysteroid dehydrogenase activity in unfixed tissue sections of rat ovary has been described. The method depends on the oxidation of dehydroepiandrosterone (DHEA) and uses nitroblue tetrazolium as the final electron acceptor. Although the dehydrogenase is not a soluble enzyme, polyvinyl alcohol is included in the reaction medium to allow the use of a high substrate concentration whilst employing a low concentration (5%) of dimethyl formamide. The enzyme is equally dependent on NAD+ or NADP+ for its activity and this activity is significantly enhanced by the presence of cyanide. The NADP+ dependence is not abolished by inhibiting nonspecific alkaline phomonoesterase. The activity of delta5,3beta-hydroxysteroid dehydrogenase is completely dependent on a functional sulphydryl group. Furthermore, the enzyme activity is totally inhibited in the presence of a steroid substrate analogue at 10(-4) M.

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Year:  1979        PMID： 34585     DOI： 10.1007/bf00689609

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  41 in total

1.  3-BETA-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN THE MOUSE LEYDIG CELL.

Authors:  A H BAILLIE; K GRIFFITHS
Journal:  J Endocrinol       Date:  1964-04       Impact factor: 4.286

2.  A histochemical study of 3(beta)-sterol dehydrogenase in the adrenal cortex of the developing mouse.

Authors:  J M ALLEN
Journal:  Anat Rec       Date:  1960-05

3.  Enzymic isomerization of delta5-3-ketosteroids.

Authors:  P TALALAY; V S WANG
Journal:  Biochim Biophys Acta       Date:  1955-10

4.  5-ene-3beta-hydroxysteroid dehydrogenase of human and bovine adrenocortical endoplasmic reticulum: solubilization and fractionation.

Authors:  A R Eastman; A M Neville
Journal:  J Endocrinol       Date:  1977-02       Impact factor: 4.286

5.  Site specificity of bovine adrenal 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase.

Authors:  S G Cheatum; A W Douville; J C Warren
Journal:  Biochim Biophys Acta       Date:  1967-02-14

6.  Specificity in steroid histochemistry, with special reference to the use of steroid solvents. Distribution of 11-beta-Hydroxysteroiddehydrogenase in kidney and thymus from the mouse.

Authors:  P E Hoyer; H Andersen
Journal:  Histochemie       Date:  1970

7.  New synthetic steroids which inhibit rat gonadal steroidogenic enzymes both in vitro and in vivo.

Authors:  A S Goldman; J A Gustafsson; S A Gustafsson
Journal:  Acta Endocrinol (Copenh)       Date:  1973-05

8.  [Activity and localization of various steroid dehydrogenases (StDH) during placentation in rats].

Authors:  W Schmidt; D Wendler; W Gabler
Journal:  Acta Histochem       Date:  1970       Impact factor: 2.479

9.  Improvements in technique for the histochemical demonstration of 3-beta and 17-beta hydroxysteroid dehydrogenase in human testis.

Authors:  H C Morse; C G Heller
Journal:  Histochemie       Date:  1973-06-29

10.  Metabolism of progesterone-4-C-14 and pregnenolone-7alpha-H3 by human adrenal tissue. Formation of 16alpha-hydroxyprogesterone-C-14, corticosterone-C-14, and cortisol-C-14-H3.

Authors:  I WELIKY; L L ENGEL
Journal:  J Biol Chem       Date:  1963-04       Impact factor: 5.157
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  14 in total

1.  Ultrastructural and histochemical study on the interrenal cells of the male stickleback (Gasterosteus aculeatus, Teleostea), in relation to the reproductive annual cycle.

Authors:  A Civinini; D Padula; V P Gallo
Journal:  J Anat       Date:  2001-09       Impact factor: 2.610

Review 2.  A review of the evolution of viviparity in squamate reptiles: the past, present and future role of molecular biology and genomics.

Authors:  Bridget F Murphy; Michael B Thompson
Journal:  J Comp Physiol B       Date:  2011-05-15       Impact factor: 2.200

Review 3.  Polyvinyl alcohol and other tissue protectants in enzyme histochemistry: a consumer's guide.

Authors:  C J Van Noorden; I M Vogels
Journal:  Histochem J       Date:  1989-07

4.  The application of continuous monitoring microdensitometry to an analysis of NAD+ binding and 3 beta-hydroxy-delta 5-steroid dehydrogenase activity in the regressing corpus luteum of the pro-oestrous rat ovary.

Authors:  M Gordon; W R Robertson
Journal:  Histochem J       Date:  1986-01

5.  Reaction rate studies of glucose-6-phosphate dehydrogenase in rat tracheal epithelium; the effect of section thickness.

Authors:  R G Butcher
Journal:  Histochemistry       Date:  1984

6.  The use of an inexpensive, general purpose microcomputer in quantitative cytochemistry.

Authors:  M T Smith; E D Wills; K Drew; C Maxwell; J R Daly; S C Reader; W R Robertson
Journal:  Histochemistry       Date:  1980

7.  The determination by microdensitometry of the initial maximum velocity rate of rat ovarian 3 beta hydroxysteroid dehydrogenase activity.

Authors:  W R Robertson; R J Earnshaw; A Lambert
Journal:  Histochemistry       Date:  1982

8.  A quantitative study of N-acetyl-beta-glucosaminidase activity in unfixed tissue sections of the guinea-pig thyroid gland.

Authors:  W R Robertson
Journal:  Histochem J       Date:  1980-01

9.  The influence on enzyme activity of storage of tissue blocks at -70 degrees C.

Authors:  B Henderson; N Loveridge; W R Robertson; M T Smith
Journal:  Histochemistry       Date:  1981

10.  Chick gonadogenesis following early surgical bursectomy. I. Histological, morphometric and histochemical data on the embryonic left ovary.

Authors:  A Civinini; S Petrucci; V P Gallo
Journal:  Anat Embryol (Berl)       Date:  1993-06
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