Myoinositol uptake by four cultured mammalian cell lines.

The uptake of myo-[2-3H]inositol by mouse neuroblastoma, human Y79 retinoblastoma, human HL60, and bovine pulmonary artery endothelial cells occurs by a saturable, Na+-dependent and partially energy-dependent mechanism. Inositol uptake by all four cell lines occurred by both a high-and low-affinity system. The kinetic parameters for the high-affinity uptake systems were similar for all four cell lines. These data suggest that all four of these diverse cell lines have similar inositol transport systems and probably rely on extracellular inositol for anabolic processes.