Literature DB >> 34562241

An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched.

Wonhwa Cho1, Arthur Ralko2, Ashutosh Sharma2.   

Abstract

We recently developed a simultaneous in situ quantitative imaging technique for cholesterol in both leaflets of the plasma membrane of mammalian cells. This ratiometric fluorescence technique allows real-time monitoring of the cholesterol transporter activity of plasma membrane-resident proteins in living cells. When applied to the hedgehog signaling system, it enables direct quantitative measurement of the cholesterol transporter activity of Patched1 and the effect of the hedgehog ligand on this activity.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cholesterol quantification; Cholesterol transporter; Fluorescence assay; Patched

Mesh：

Substances：

Year:  2022        PMID： 34562241     DOI： 10.1007/978-1-0716-1701-4_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  20 in total

1.  Photostable and Orthogonal Solvatochromic Fluorophores for Simultaneous In Situ Quantification of Multiple Cellular Signaling Molecules.

Authors:  Ashutosh Sharma; Jiachen Sun; Indira Singaram; Arthur Ralko; Daesung Lee; Wonhwa Cho
Journal:  ACS Chem Biol       Date:  2020-06-25       Impact factor: 5.100

Review 2.  Modulation of membrane function by cholesterol.

Authors:  P L Yeagle
Journal:  Biochimie       Date:  1991-10       Impact factor: 4.079

3.  Simultaneous in situ quantification of two cellular lipid pools using orthogonal fluorescent sensors.

Authors:  Shu-Lin Liu; Ren Sheng; Matthew J O'Connor; Yang Cui; Youngdae Yoon; Svetlana Kurilova; Daesung Lee; Wonhwa Cho
Journal:  Angew Chem Int Ed Engl       Date:  2014-10-24       Impact factor: 15.336

4.  The effect of sterol structure on the permeability of lipomes to glucose, glycerol and Rb + .

Authors:  R A Demel; K R Bruckdorfer; L L van Deenen
Journal:  Biochim Biophys Acta       Date:  1972-01-17

5.  Quantitative Lipid Imaging Reveals a New Signaling Function of Phosphatidylinositol-3,4-Bisphophate: Isoform- and Site-Specific Activation of Akt.

Authors:  Shu-Lin Liu; Zhi-Gang Wang; Yusi Hu; Yao Xin; Indira Singaram; Sukhamoy Gorai; Xin Zhou; Yoonjung Shim; Jung-Hyun Min; Liang-Wei Gong; Nissim Hay; Jin Zhang; Wonhwa Cho
Journal:  Mol Cell       Date:  2018-08-30       Impact factor: 17.970

Review 6.  Role of cholesterol and lipid organization in disease.

Authors:  Frederick R Maxfield; Ira Tabas
Journal:  Nature       Date:  2005-12-01       Impact factor: 49.962

7.  Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol.

Authors:  Shu-Lin Liu; Ren Sheng; Jae Hun Jung; Li Wang; Ewa Stec; Matthew J O'Connor; Seohyoen Song; Rama Kamesh Bikkavilli; Robert A Winn; Daesung Lee; Kwanghee Baek; Kazumitsu Ueda; Irena Levitan; Kwang-Pyo Kim; Wonhwa Cho
Journal:  Nat Chem Biol       Date:  2016-12-26       Impact factor: 15.040

Review 8.  Cellular cholesterol trafficking and compartmentalization.

Authors:  Elina Ikonen
Journal:  Nat Rev Mol Cell Biol       Date:  2008-02       Impact factor: 94.444

Review 9.  Lipid rafts as a membrane-organizing principle.

Authors:  Daniel Lingwood; Kai Simons
Journal:  Science       Date:  2010-01-01       Impact factor: 47.728

10.  In situ quantitative imaging of cellular lipids using molecular sensors.

Authors:  Youngdae Yoon; Park J Lee; Svetlana Kurilova; Wonhwa Cho
Journal:  Nat Chem       Date:  2011-10-09       Impact factor: 24.427
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