Jessica Hentilä1, Noora Laakamaa1, Timo Sorsa2, Jukka Meurman1, Hanna Välimaa3, Sakari Nikinmaa4, Esko Kankuri4, Tuomas Tauriainen5, Tommi Pätilä6. 1. Department of Oral and Maxillofacial Surgery, University of Helsinki and Helsinki University Hospital, Finland. 2. Department of Oral and Maxillofacial Surgery, University of Helsinki and Helsinki University Hospital, Finland and Department of Oral Medicine, Karolinska Institute, Huddinge, Sweden. 3. Department of Oral and Maxillofacial Surgery and Department of Virology, University of Helsinki and Helsinki Hospital, Finland. 4. Department of Neuroscience and Biomedical Engineering, Aalto University, Finland. 5. Heart and Lung Center, Meilahti Hospital, Helsinki, Finland. 6. Department of Congenital Heart Surgery and Organ Transplantation, New Children's Hospital, University of Helsinki, Finland.
Abstract
PURPOSE: During cancer treatment, oral mucositis due to radiotherapy or chemotherapy often leads to disruption of the oral mucosa, enabling microbes to invade bloodstream. Viridans streptococcal species are part of the healthy oral microbiota but can be frequently isolated from the blood of neutropenic patients. We have previously shown the antibacterial efficacy of dual-light, the combination of antibacterial blue light (aBL) and indocyanine green photodynamic therapy (aPDT). METHODS: Here, we investigated the dual-light antibacterial action against four-day Streptococcus oralis biofilm. In addition, while keeping the total radiant exposure constant at 100J/cm2, we investigated the effect of changing the different relative light energies of aBL and aPDT to the antibacterial potential. RESULTS: The dual-light had a significant antibacterial effect in all the tested combinations. CONCLUSION: Dual-light can be used as an effective disinfectant against S. oralis biofilm.
PURPOSE: During cancer treatment, oral mucositis due to radiotherapy or chemotherapy often leads to disruption of the oral mucosa, enabling microbes to invade bloodstream. Viridans streptococcal species are part of the healthy oral microbiota but can be frequently isolated from the blood of neutropenic patients. We have previously shown the antibacterial efficacy of dual-light, the combination of antibacterial blue light (aBL) and indocyanine green photodynamic therapy (aPDT). METHODS: Here, we investigated the dual-light antibacterial action against four-day Streptococcus oralis biofilm. In addition, while keeping the total radiant exposure constant at 100J/cm2, we investigated the effect of changing the different relative light energies of aBL and aPDT to the antibacterial potential. RESULTS: The dual-light had a significant antibacterial effect in all the tested combinations. CONCLUSION: Dual-light can be used as an effective disinfectant against S. oralis biofilm.
Authors: Hanna Lähteenmäki; Tommi Pätilä; Ismo T Räisänen; Esko Kankuri; Taina Tervahartiala; Timo Sorsa Journal: Curr Issues Mol Biol Date: 2022-03-08 Impact factor: 2.976