Literature DB >> 34558006

Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord.

Luc Bauchet1,2,3, Gaetan Poulen4,5, Nicolas Lonjon4, Florence Vachiery-Lahaye6, Emmanuel Bourinet7, Florence Evelyne Perrin8, Jean-Philippe Hugnot7.   

Abstract

We demonstrated the presence of neural stem cells and/or progenitor cells in the adult human spinal cord. This chapter provides materials and methods to harvest high-quality samples of thoracolumbar, lumbar, and sacral adult human spinal cord and human dorsal root ganglia isolated from brain-dead patients who had agreed before passing to donate their bodies to science for therapeutic and scientific advances. The methods to culture precursor cells from the adult human spinal cord are also described.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Adult human spinal cord; Organ donation; Precursor cells; Stem cells

Mesh：

Year:  2022        PMID： 34558006     DOI： 10.1007/978-1-0716-1783-0_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Cellular organization of the central canal ependymal zone, a niche of latent neural stem cells in the adult mammalian spinal cord.

Authors:  L K Hamilton; M K V Truong; M R Bednarczyk; A Aumont; K J L Fernandes
Journal:  Neuroscience       Date:  2009-09-09       Impact factor: 3.590

2.  Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro.

Authors:  C Dromard; H Guillon; V Rigau; C Ripoll; J C Sabourin; F E Perrin; F Scamps; S Bozza; P Sabatier; N Lonjon; H Duffau; F Vachiery-Lahaye; M Prieto; C Tran Van Ba; L Deleyrolle; A Boularan; K Langley; M Gaviria; A Privat; J P Hugnot; L Bauchet
Journal:  J Neurosci Res       Date:  2008-07       Impact factor: 4.164

Review 3.  Role of endogenous neural stem cells in spinal cord injury and repair.

Authors:  Moa Stenudd; Hanna Sabelström; Jonas Frisén
Journal:  JAMA Neurol       Date:  2015-02       Impact factor: 18.302

Review 4.  The spinal ependymal zone as a source of endogenous repair cells across vertebrates.

Authors:  Catherina G Becker; Thomas Becker; Jean-Philippe Hugnot
Journal:  Prog Neurobiol       Date:  2018-04-09       Impact factor: 11.685

5.  Anatomical study of serotonergic innervation and 5-HT(1A) receptor in the human spinal cord.

Authors:  F E Perrin; Y N Gerber; M Teigell; N Lonjon; G Boniface; L Bauchet; J J Rodriguez; J P Hugnot; A M Privat
Journal:  Cell Death Dis       Date:  2011-10-13       Impact factor: 8.469

6.  Isolation of mineralizing Nestin+ Nkx6.1+ vascular muscular cells from the adult human spinal cord.

Authors:  Daria Mamaeva; Chantal Ripoll; Claire Bony; Marisa Teigell; Florence E Perrin; Bernard Rothhut; Ivan Bieche; Rosette Lidereau; Alain Privat; Valérie Rigau; Hélène Guillon; Florence Vachiery-Lahaye; Daniele Noel; Luc Bauchet; Jean-Philippe Hugnot
Journal:  BMC Neurosci       Date:  2011-10-10       Impact factor: 3.288
  6 in total

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