Literature DB >> 34542848

Imaging the Actin Cytoskeleton in Live Budding Yeast Cells.

Cierra N Sing1, Emily J Yang1, Theresa C Swayne2, Ryo Higuchi-Sanabria3, Catherine A Tsang1, Istvan R Boldogh1,2, Liza A Pon4,5.   

Abstract

Although budding yeast, Saccharomyces cerevisiae, is widely used as a model organism in biological research, studying cell biology in yeast was hindered due to its small size, rounded morphology, and cell wall. However, with improved techniques, researchers can acquire high-resolution images and carry out rapid multidimensional analysis of a yeast cell. As a result, imaging in yeast has emerged as an important tool to study cytoskeletal organization, function, and dynamics. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in live yeast cells.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Actin; Cytoskeleton; Deconvolution; Fluorescent proteins; Live cell imaging; Microscopy; Ratio imaging; Vital staining; Yeast

Mesh：

Substances：

Year:  2022        PMID： 34542848     DOI： 10.1007/978-1-0716-1661-1_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  59 in total

Review 1.  Mechanisms of polarized growth and organelle segregation in yeast.

Authors:  David Pruyne; Aster Legesse-Miller; Lina Gao; Yuqing Dong; Anthony Bretscher
Journal:  Annu Rev Cell Dev Biol       Date:  2004       Impact factor: 13.827

2.  New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae.

Authors:  Robert Gauss; Mark Trautwein; Thomas Sommer; Anne Spang
Journal:  Yeast       Date:  2005-01-15       Impact factor: 3.239

3.  Actin cable dynamics in budding yeast.

Authors:  Hyeong-Cheol Yang; Liza A Pon
Journal:  Proc Natl Acad Sci U S A       Date:  2002-01-22       Impact factor: 11.205

4.  A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.

Authors:  U Gueldener; J Heinisch; G J Koehler; D Voss; J H Hegemann
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

Review 5.  The ARP2/3 complex: an actin nucleator comes of age.

Authors:  Erin D Goley; Matthew D Welch
Journal:  Nat Rev Mol Cell Biol       Date:  2006-10       Impact factor: 94.444

Review 6.  Actin assembly and endocytosis: from yeast to mammals.

Authors:  Asa E Y Engqvist-Goldstein; David G Drubin
Journal:  Annu Rev Cell Dev Biol       Date:  2003       Impact factor: 13.827

7.  Actin dynamics affect mitochondrial quality control and aging in budding yeast.

Authors:  Ryo Higuchi; Jason D Vevea; Theresa C Swayne; Robert Chojnowski; Vanessa Hill; Istvan R Boldogh; Liza A Pon
Journal:  Curr Biol       Date:  2013-11-21       Impact factor: 10.834

8.  Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast.

Authors:  Thomas M Huckaba; Thomas Lipkin; Liza A Pon
Journal:  J Cell Biol       Date:  2006-12-18       Impact factor: 10.539

9.  Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.

Authors:  A E Adams; J R Pringle
Journal:  J Cell Biol       Date:  1984-03       Impact factor: 10.539

10.  β2 adrenergic receptor fluorescent protein fusions traffic to the plasma membrane and retain functionality.

Authors:  Jaclyn Bubnell; Patrick Pfister; Maria L Sapar; Matthew E Rogers; Paul Feinstein
Journal:  PLoS One       Date:  2013-09-23       Impact factor: 3.240
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