| Literature DB >> 34542752 |
A A Kubanov1, D G Deryabin2, M V Shpilevaya2, A E Karamova2, A A Nikonorov2, E N Larina3, T K Aliev4, D A Dolgikh3,4, T V Bobik3, I V Smirnov3, A G Gabibov3, M P Kirpichnikov3,4.
Abstract
A method for the analysis of the epitope specificity of auto-reactive antibodies to desmoglein 3 (Dsg3) using competitive ELISA has been developed. It is based on a two-stage solid-phase ELISA with initial "depletion" of auto-reactive antibodies against the studied epitope and subsequent quantitative assessment of antibodies against full-length extracellular domain Dsg3. The proposed approach for assessing the specificity of the autoimmune response in patients with pemphigus vulgaris can provide in the future the possibility to personalize the therapy using plasmapheresis by preliminary selection of the antigenic composition of the extracorporeal immunosorbent.Entities:
Keywords: ELISA; autoantibodies; autoimmunity; desmoglein 3; pemphigus vulgaris; specificity
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Year: 2021 PMID： 34542752 DOI： 10.1007/s10517-021-05254-9
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804