Literature DB >> 3454126

Immobilization of proteins (antibodies) to supports with a constantly high retention of immunoreactivity involving the formation of a Schiff-base and subsequent reduction to a secondary amine with sodium borohydride or sodium cyanoborohydride has been problematical.

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Year:  1987        PMID： 3454126     DOI： 10.1007/BF02801317

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


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  11 in total

1.  Factors affecting the properties of insolubilized anitbodies.

Authors:  S Comoglio; A Massaglia; E Rolleri; U Rosa
Journal:  Biochim Biophys Acta       Date:  1976-02-20

2.  Reductive alkylation of proteins with aromatic aldehydes and sodium cyanoborohydride.

Authors:  M Friedman; L D Williams; M S Masri
Journal:  Int J Pept Protein Res       Date:  1974

3.  The direct coupling of oligosaccharides to proteins and derivatized gels.

Authors:  G R Gray
Journal:  Arch Biochem Biophys       Date:  1974-07       Impact factor: 4.013

4.  Labeling of proteins by reductive methylation using sodium cyanoborohydride.

Authors:  N Jentoft; D G Dearborn
Journal:  J Biol Chem       Date:  1979-06-10       Impact factor: 5.157

5.  Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride.

Authors:  D Dottavio-Martin; J M Ravel
Journal:  Anal Biochem       Date:  1978-07-01       Impact factor: 3.365

6.  Some monoclonal antibodies raised with a native protein bind preferentially to the denatured antigen.

Authors:  B Friguet; L Djavadi-Ohaniance; M E Goldberg
Journal:  Mol Immunol       Date:  1984-07       Impact factor: 4.407

7.  A rapid solution-phase screening technique for hybridoma culture supernatants using radiolabeled antigen and a solid-phase immunoadsorbent.

Authors:  K F Miller; D J Bolt; R A Goldsby
Journal:  J Immunol Methods       Date:  1983-05-13       Impact factor: 2.303

8.  The influence of methods of production, purification and storage of monoclonal antibodies upon their observed specificities.

Authors:  P A Underwood; P A Bean
Journal:  J Immunol Methods       Date:  1985-06-25       Impact factor: 2.303

9.  Effect of borohydride reduction on antibodies.

Authors:  L Peng; G J Calton; J W Burnett
Journal:  Appl Biochem Biotechnol       Date:  1987-03       Impact factor: 2.926

10.  Alternative reducing agents for reductive methylation of amino groups in proteins.

Authors:  K F Geoghegan; J C Cabacungan; H B Dixon; R E Feeney
Journal:  Int J Pept Protein Res       Date:  1981-03
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