Literature DB >> 34541245

Mating Based Split-ubiquitin Assay for Detection of Protein Interactions.

Wijitra Horaruang1, Ben Zhang1.   

Abstract

The mating based split-ubiquitin (mbSUS) assay is an alternative method to the classical yeast two-hybrid system with a number of advantages. The mbSUS assay relies on the ubiquitin-degradation pathway as a sensor for protein-protein interactions, and it is suitable for the determination of interactions between full-length proteins that are cytosolic or membrane-bound. Here we describe the mbSUS assay protocol which has been used for detecting the interaction between K+ channel and SNARE proteins ( Grefen et al., 2010 and 2015; Zhang et al., 2015 and 2016).
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Gateway; Mating; Protein-protein interaction; Split-ubiquitin; Yeast; mbSUS

Year:  2017        PMID： 34541245      PMCID： PMC8410247          DOI： 10.21769/BioProtoc.2258

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions.

Authors:  Petr Obrdlik; Mohamed El-Bakkoury; Tanja Hamacher; Corinna Cappellaro; Cristina Vilarino; Carola Fleischer; Heinz Ellerbrok; Richard Kamuzinzi; Valérie Ledent; Damien Blaudez; Dale Sanders; Jose L Revuelta; Eckhard Boles; Bruno André; Wolf B Frommer
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-06       Impact factor: 11.205

Review 2.  Molecular basis for specificity of nuclear import and prediction of nuclear localization.

Authors:  Mary Marfori; Andrew Mynott; Jonathan J Ellis; Ahmed M Mehdi; Neil F W Saunders; Paul M Curmi; Jade K Forwood; Mikael Bodén; Bostjan Kobe
Journal:  Biochim Biophys Acta       Date:  2010-10-25

3.  The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS).

Authors:  Christopher Grefen; Petr Obrdlik; Klaus Harter
Journal:  Methods Mol Biol       Date:  2009

4.  A novel motif essential for SNARE interaction with the K(+) channel KC1 and channel gating in Arabidopsis.

Authors:  Christopher Grefen; Zhonghua Chen; Annegret Honsbein; Naomi Donald; Adrian Hills; Michael R Blatt
Journal:  Plant Cell       Date:  2010-09-30       Impact factor: 11.277

5.  Do calcineurin B-like proteins interact independently of the serine threonine kinase CIPK23 with the K+ channel AKT1? Lessons learned from a ménage à trois.

Authors:  Christopher Grefen; Michael R Blatt
Journal:  Plant Physiol       Date:  2012-05-01       Impact factor: 8.340

6.  VAMP721 Conformations Unmask an Extended Motif for K+ Channel Binding and Gating Control.

Authors:  Ben Zhang; Rucha Karnik; Sakharam Waghmare; Naomi Donald; Michael R Blatt
Journal:  Plant Physiol       Date:  2016-11-07       Impact factor: 8.340

7.  The Arabidopsis R-SNARE VAMP721 Interacts with KAT1 and KC1 K+ Channels to Moderate K+ Current at the Plasma Membrane.

Authors:  Ben Zhang; Rucha Karnik; Yizhou Wang; Niklas Wallmeroth; Michael R Blatt; Christopher Grefen
Journal:  Plant Cell       Date:  2015-05-22       Impact factor: 11.277

8.  A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion.

Authors:  Christopher Grefen; Rucha Karnik; Emily Larson; Cécile Lefoulon; Yizhou Wang; Sakharam Waghmare; Ben Zhang; Adrian Hills; Michael R Blatt
Journal:  Nat Plants       Date:  2015-08-03       Impact factor: 15.793
  8 in total
  1 in total

1.  Identification and Characterization of Shaker K+ Channel Gene Family in Foxtail Millet (Setaria italica) and Their Role in Stress Response.

Authors:  Ben Zhang; Yue Guo; Hui Wang; Xiaoxia Wang; Mengtao Lv; Pu Yang; Lizhen Zhang
Journal:  Front Plant Sci       Date:  2022-06-09       Impact factor: 6.627
  1 in total

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