Literature DB >> 34541243

Preparation of Everted Membrane Vesicles from Escherichia coli Cells.

Marina Verkhovskaya1.   

Abstract

The protocol for obtaining electrically sealed membrane vesicles from E. coli cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by monitoring the electric potential generated by these pumps.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Escherichia coli; Membrane vesicles; Proton pumping

Year:  2017        PMID： 34541243      PMCID： PMC8410345          DOI： 10.21769/BioProtoc.2254

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

1.  Measurement of the membrane potential generated by complex I in submitochondrial particles.

Authors:  A Ghelli; B Benelli; M D Esposti
Journal:  J Biochem       Date:  1997-04       Impact factor: 3.387

2.  Electrophysiological study with oxonol VI of passive NO3- transport by isolated plant root plasma membrane.

Authors:  P Pouliquin; J Grouzis; R Gibrat
Journal:  Biophys J       Date:  1999-01       Impact factor: 4.033

3.  Conserved lysine residues of the membrane subunit NuoM are involved in energy conversion by the proton-pumping NADH:ubiquinone oxidoreductase (Complex I).

Authors:  Liliya Euro; Galina Belevich; Michael I Verkhovsky; Mårten Wikström; Marina Verkhovskaya
Journal:  Biochim Biophys Acta       Date:  2008-06-09

4.  Probing the mechanistic role of the long α-helix in subunit L of respiratory Complex I from Escherichia coli by site-directed mutagenesis.

Authors:  Galina Belevich; Juho Knuuti; Michael I Verkhovsky; Mårten Wikström; Marina Verkhovskaya
Journal:  Mol Microbiol       Date:  2011-11-07       Impact factor: 3.501
  4 in total

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