| Literature DB >> 34541221 |
Zach Geisterfer1, Gin Tezuka1, Li Tao1.
Abstract
Kinesins play a role in organizing the mitotic spindle through the crosslinking of microtubules (MTs), made possible through binding sites at opposite ends of the holoenzyme. Here, we developed a method to test kinesin MT crosslinking action under physiological conditions.Entities:
Keywords: DEAE-Dextran coated glass chamber; Fluorescence microtubules; Kinesin motors; Physiological [ATP]; in vitro bundling assay
Year: 2017 PMID： 34541221 PMCID： PMC8410401 DOI： 10.21769/BioProtoc.2217
Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325