Literature DB >> 34541218

Gliding Assay to Analyze Microtubule-based Motor Protein Dynamics.

Albert Shim1, Gin Tezuka1, Luke Kupcha1, Li Tao1.   

Abstract

The purpose of this protocol is to provide an updated method of performing microtubule gliding assays and visualizing it using fluorescence microscopy.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Glass chamber; Gliding assay; Mitotic motor; Polarity-marked microtubules; in vitro motility

Year:  2017        PMID： 34541218      PMCID： PMC8410373          DOI： 10.21769/BioProtoc.2210

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

1.  The load dependence of kinesin's mechanical cycle.

Authors:  C M Coppin; D W Pierce; L Hsu; R D Vale
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

2.  A homotetrameric kinesin-5, KLP61F, bundles microtubules and antagonizes Ncd in motility assays.

Authors:  Li Tao; Alex Mogilner; Gul Civelekoglu-Scholey; Roy Wollman; James Evans; Henning Stahlberg; Jonathan M Scholey
Journal:  Curr Biol       Date:  2006-12-05       Impact factor: 10.834

Review 3.  Purification and assay of mitotic motors.

Authors:  Li Tao; Jonathan M Scholey
Journal:  Methods       Date:  2010-01-22       Impact factor: 3.608

4.  Tum/RacGAP functions as a switch activating the Pav/kinesin-6 motor.

Authors:  Li Tao; Barbara Fasulo; Brandt Warecki; William Sullivan
Journal:  Nat Commun       Date:  2016-04-19       Impact factor: 14.919
  4 in total

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