| Literature DB >> 34541215 |
Abstract
The activity of the endo-β-1,4-galactanase GanB from B. subtilis on the high molecular weight β-1,4-galactan was determined quantitatively by the measurement of the increase of the reducing power or with the dyed substrate Azo-galactan. The generated degradation products were analyzed using thinlayer-chromatography (TLC) or high-performance anion-exchange chromatography (HPAEC).Entities:
Keywords: GanB from Bacillus subtilis; AZCL-galactan; Endo-β-galactanase; Galactan; Galactanase-assay; Galacto-oligosaccharides
Year: 2017 PMID： 34541215 PMCID： PMC8410334 DOI： 10.21769/BioProtoc.2206
Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325