Literature DB >> 34541208

Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition.

Susan M Carnachan1, Simon F R Hinkley1.   

Abstract

Heparan sulfate (HS) is purified from complex matrices and often not fully characterised to validate its assignment. The characterisation of heparins and heparan sulfates through enzymatic depolymerisation and subsequent strong anion-exchange high performance liquid chromatography (SAX-HPLC) analysis and quantitation of the resulting disaccharides is a critical tool for assessing the structural composition of this class of compound. This protocol details a methodology to reproducibly determine the disaccharide composition of heparan sulfate by enzymatic depolymerisation and SAX-HPLC analysis. A complementary method for identification and characterisation of heparan sulfate can be found at Carnachan and Hinkley (2017).
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Chemical characterization; Enzymatic depolymerisation; Glycosaminoglycan’s; HPLC; Heparan sulfate

Year:  2017        PMID： 34541208      PMCID： PMC8410264          DOI： 10.21769/BioProtoc.2197

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

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Authors:  Mark A Skidmore; Scott E Guimond; Audrey F Dumax-Vorzet; Edwin A Yates; Jeremy E Turnbull
Journal:  Nat Protoc       Date:  2010-12-02       Impact factor: 13.491

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3.  Determining the extent of heparan sulfate depolymerisation following heparin lyase treatment.

Authors:  Susan M Carnachan; Tracey J Bell; Ian M Sims; Raymond A A Smith; Victor Nurcombe; Simon M Cool; Simon F R Hinkley
Journal:  Carbohydr Polym       Date:  2016-07-07       Impact factor: 9.381

4.  High sensitivity separation and detection of heparan sulfate disaccharides.

Authors:  M A Skidmore; S E Guimond; A F Dumax-Vorzet; A Atrih; E A Yates; J E Turnbull
Journal:  J Chromatogr A       Date:  2006-10-10       Impact factor: 4.759

5.  An integrated approach using orthogonal analytical techniques to characterize heparan sulfate structure.

Authors:  Daniela Beccati; Miroslaw Lech; Jennifer Ozug; Nur Sibel Gunay; Jing Wang; Elaine Y Sun; Joël R Pradines; Victor Farutin; Zachary Shriver; Ganesh V Kaundinya; Ishan Capila
Journal:  Glycoconj J       Date:  2016-10-22       Impact factor: 2.916

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Journal:  Anal Chem       Date:  2016-06-15       Impact factor: 6.986
  6 in total
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