Literature DB >> 34541207

Heparan Sulfate Identification and Characterisation: Method I.Heparan Sulfate Identification by NMR Analysis.

Susan M Carnachan1, Simon F R Hinkley1.   

Abstract

Heparin and heparan sulfate (HS) may be purified from complex biological matrices and are often isolated in sub-milligram quantities but not unequivocally identified by spectroscopic means. This protocol details a methodology to rapidly assess the gross compositional features and approximate purity of HS by 1H nuclear magnetic resonance. A complimentary method for identification and characterisation of heparan sulfate can be found at Carnachan and Hinkley (2017).
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Chemical characterization; Glycosaminoglycan’s; Heparan sulfate; NMR

Year:  2017        PMID： 34541207      PMCID： PMC8410356          DOI： 10.21769/BioProtoc.2196

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  High-specific-activity 35S-labeled heparan sulfate prepared from cultured cells.

Authors:  N W Shworak
Journal:  Methods Mol Biol       Date:  2001

2.  Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events.

Authors:  Marco Guerrini; Daniela Beccati; Zachary Shriver; Annamaria Naggi; Karthik Viswanathan; Antonella Bisio; Ishan Capila; Jonathan C Lansing; Sara Guglieri; Blair Fraser; Ali Al-Hakim; Nur Sibel Gunay; Zhenqing Zhang; Luke Robinson; Lucinda Buhse; Moheb Nasr; Janet Woodcock; Robert Langer; Ganesh Venkataraman; Robert J Linhardt; Benito Casu; Giangiacomo Torri; Ram Sasisekharan
Journal:  Nat Biotechnol       Date:  2008-04-23       Impact factor: 54.908

3.  Assay of possible economically motivated additives or native impurities levels in heparin by 1H NMR, SAX-HPLC, and anticoagulation time approaches.

Authors:  David A Keire; Daniel J Mans; Hongping Ye; Richard E Kolinski; Lucinda F Buhse
Journal:  J Pharm Biomed Anal       Date:  2010-02-20       Impact factor: 3.935

4.  Complex glycosaminoglycans: profiling substitution patterns by two-dimensional nuclear magnetic resonance spectroscopy.

Authors:  Marco Guerrini; Annamaria Naggi; Sara Guglieri; Roberto Santarsiero; Giangiacomo Torri
Journal:  Anal Biochem       Date:  2005-02-01       Impact factor: 3.365

Review 5.  Lessons learned from the contamination of heparin.

Authors:  Haiying Liu; Zhenqing Zhang; Robert J Linhardt
Journal:  Nat Prod Rep       Date:  2009-01-19       Impact factor: 13.423

6.  Determining the extent of heparan sulfate depolymerisation following heparin lyase treatment.

Authors:  Susan M Carnachan; Tracey J Bell; Ian M Sims; Raymond A A Smith; Victor Nurcombe; Simon M Cool; Simon F R Hinkley
Journal:  Carbohydr Polym       Date:  2016-07-07       Impact factor: 9.381
  6 in total
  1 in total

1.  Sulfonate Hydrogel-siRNA Conjugate Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells by Controlled Gene Silencing and Activation of BMP Signaling.

Authors:  Soyon Kim; Jiabing Fan; Chung-Sung Lee; Chen Chen; Min Lee
Journal:  ACS Appl Bio Mater       Date:  2021-05-27
  1 in total

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