Literature DB >> 34541187

In vivo Priming of T Cells with in vitro Pulsed Dendritic Cells: Popliteal Lymph Node Assay.

Songjie Cai1, Masayuki Fujino1,2, Lina Lu3, Xiao-Kang Li1.   

Abstract

One-way mixed lymphocyte reaction (MLR) is a classic tool to measure how T cells react to external stimuli. However, MLR is an in vitro reaction system, which shows different response intensity compared with in vivo trails sometimes due to the lack of cytokines, tissue matrix and other immune response associated factors. The following popliteal lymph node assay (PLNA) protocol is designed to test the T cells antigen-specific reaction in vivo by using ovalbumin (OVA) specific reacted transgenic mouse OT-1 and OT-2.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Antigen presenting cells (APC); Dendritic cells (DC); Flow cytometry; Mixed lymphocyte reaction (MLR); OT-1; OT-2; Ovalbumin antigen; Popliteal lymph node assay (PLNA)

Year:  2017        PMID： 34541187      PMCID： PMC8413589          DOI： 10.21769/BioProtoc.2531

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

1.  Permanent acceptance of mouse cardiac allografts with CD40 siRNA to induce regulatory myeloid cells by use of a novel polysaccharide siRNA delivery system.

Authors:  Q Zhang; N Ichimaru; S Higuchi; S Cai; J Hou; M Fujino; N Nonomura; M Kobayashi; H Ando; A Uno; K Sakurai; S Mochizuki; Y Adachi; N Ohno; H Zou; J Xu; X-K Li; S Takahara
Journal:  Gene Ther       Date:  2015-01-08       Impact factor: 5.250

2.  5-Aminolevulinic acid with ferrous iron induces permanent cardiac allograft acceptance in mice via induction of regulatory cells.

Authors:  Jiangang Hou; Qi Zhang; Masayuki Fujino; Songjie Cai; Hidenori Ito; Kiwamu Takahashi; Fuminori Abe; Motowo Nakajima; Tohru Tanaka; Jinhua Xu; Hejian Zou; Qiang Ding; Xiao-Kang Li
Journal:  J Heart Lung Transplant       Date:  2014-10-02       Impact factor: 10.247

3.  All-trans retinoic acid induces arginase-1 and inducible nitric oxide synthase-producing dendritic cells with T cell inhibitory function.

Authors:  Sumantha Bhatt; Jie Qin; Carole Bennett; Shiguang Qian; John J Fung; Thomas A Hamilton; Lina Lu
Journal:  J Immunol       Date:  2014-04-30       Impact factor: 5.422

4.  Combining Exosomes Derived from Immature DCs with Donor Antigen-Specific Treg Cells Induces Tolerance in a Rat Liver Allograft Model.

Authors:  Ben Ma; Jing-Yue Yang; Wen-Jie Song; Rui Ding; Zhuo-Chao Zhang; Hong-Chen Ji; Xuan Zhang; Jian-Lin Wang; Xi-Sheng Yang; Kai-Shan Tao; Ke-Feng Dou; Xiao Li
Journal:  Sci Rep       Date:  2016-09-19       Impact factor: 4.379

5.  iPSC-Derived Regulatory Dendritic Cells Inhibit Allograft Rejection by Generating Alloantigen-Specific Regulatory T Cells.

Authors:  Songjie Cai; Jiangang Hou; Masayuki Fujino; Qi Zhang; Naotsugu Ichimaru; Shiro Takahara; Ryoko Araki; Lina Lu; Ji-Mei Chen; Jian Zhuang; Ping Zhu; Xiao-Kang Li
Journal:  Stem Cell Reports       Date:  2017-04-20       Impact factor: 7.765
  5 in total

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