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Literature DB >> 34541163

Assessment of Modulation of Protein Stability Using Pulse-chase Method.

Abstract

Pulse-chase technique is a method widely used to assess protein or mRNA stability. The principle of pulse-chase relies on labeling proteins or mRNA produced during a short period of time called 'pulse' and then following the rate of disappearance of those labeled proteins over a period of time called 'chase'. This technique thus allows quantitative analysis of modulation of protein or mRNA stability under different treatments or culturing conditions.

Entities: Chemical

Keywords: Half-life; Lysosome; MCL-1; Proteasome; Protein degradation; Protein stability; Pulse-chase; Sunitinib; mTOR

Year: 2017 PMID： 34541163 PMCID： PMC8413622 DOI： 10.21769/BioProtoc.2443

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

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References

7 in total

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Assessment of Modulation of Protein Stability Using Pulse-chase Method.

1. Pulse-chase labeling techniques for the analysis of protein maturation and degradation.

2. A novel autophagy-independent, oncosuppressive function of BECN1: Degradation of MCL1.

3. Beclin 1 restrains tumorigenesis through Mcl-1 destabilization in an autophagy-independent reciprocal manner.

4. Dual modulation of MCL-1 and mTOR determines the response to sunitinib.

Review 5. Dual inhibition of mTOR pathway and VEGF signalling in neuroendocrine neoplasms: from bench to bedside.

6. Radioactive Pulse-Chase Analysis and Immunoprecipitation.

7. The yin yang of sunitinib: One drug, two doses, and multiple outcomes.