Literature DB >> 34541151

Preparation of Crude Synaptosomal Fractions from Mouse Brains and Spinal Cords.

Oliver Wirths1.   

Abstract

The current protocol describes the preparation of crude synaptosomal fractions from mouse brain or spinal cord samples. In detail, a sequential protocol yielding crude synaptosomal and light membrane fractions is provided. This fast and easy method might be sufficient to assess the amount of synaptic proteins in down-steam applications like Western-blot or ELISA in e.g., mouse models of Alzheimer's disease or other neurodegenerative conditions.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Brain; Extraction; Fractionation; Homogenization; Synapse; Synaptosome

Year:  2017        PMID： 34541151      PMCID： PMC8413490          DOI： 10.21769/BioProtoc.2423

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


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