Literature DB >> 34541150

Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords.

Oliver Wirths1.   

Abstract

The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western blotting.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Brain; Homogenization; Protein extraction; SDS; Soluble fraction

Year:  2017        PMID： 34541150      PMCID： PMC8413590          DOI： 10.21769/BioProtoc.2422

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  Endogenous Apolipoprotein E (ApoE) Fragmentation Is Linked to Amyloid Pathology in Transgenic Mouse Models of Alzheimer's Disease.

Authors:  Anika Saul; Oliver Wirths
Journal:  Mol Neurobiol       Date:  2016-01-07       Impact factor: 5.590

2.  Immunolesion-induced loss of cholinergic projection neurones promotes β-amyloidosis and tau hyperphosphorylation in the hippocampus of triple-transgenic mice.

Authors:  W Härtig; A Saul; J Kacza; J Grosche; S Goldhammer; D Michalski; O Wirths
Journal:  Neuropathol Appl Neurobiol       Date:  2014-02       Impact factor: 8.090
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.