Literature DB >> 34541149

Polyamine and Paraquat Transport Assays in Arabidopsis Seedling and Callus.

Haoxi Chai1, Yun Shen1, Huazhong Shi1.   

Abstract

Polyamines (PAs) are polycationic compounds found in all living organisms and play crucial roles in growth and survival. We here show the 'Polyamine and paraquat (PQ) transport assay' protocol, which can be used to examine the uptake activity of PA/PQ transporters. We have used this protocol to demonstrate that PUT3 in Arabidopsis is a polyamine transporter and is able to take up spermidine and its analog paraquat.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Arabidopsis thaliana; Paraquat; Polyamine; Transport; Uptake

Year:  2017        PMID: 34541149      PMCID: PMC8413501          DOI: 10.21769/BioProtoc.2421

Source DB:  PubMed          Journal:  Bio Protoc        ISSN: 2331-8325


  5 in total

Review 1.  Polyamines and abiotic stress tolerance in plants.

Authors:  Sarvajeet Singh Gill; Narendra Tuteja
Journal:  Plant Signal Behav       Date:  2010-01

2.  Polyamine uptake by DUR3 and SAM3 in Saccharomyces cerevisiae.

Authors:  Takeshi Uemura; Keiko Kashiwagi; Kazuei Igarashi
Journal:  J Biol Chem       Date:  2007-01-11       Impact factor: 5.157

3.  Kinetic and phylogenetic analysis of plant polyamine uptake transporters.

Authors:  Vaishali Mulangi; Marcus C Chibucos; Vipaporn Phuntumart; Paul F Morris
Journal:  Planta       Date:  2012-06-19       Impact factor: 4.116

4.  Paraquat Resistant1, a Golgi-localized putative transporter protein, is involved in intracellular transport of paraquat.

Authors:  Jianyong Li; Jinye Mu; Jiaoteng Bai; Fuyou Fu; Tingting Zou; Fengying An; Jian Zhang; Hongwei Jing; Qing Wang; Zhen Li; Shuhua Yang; Jianru Zuo
Journal:  Plant Physiol       Date:  2013-03-07       Impact factor: 8.340

5.  The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability.

Authors:  Yun Shen; Qingxia Ruan; Haoxi Chai; Yongze Yuan; Wannian Yang; Junping Chen; Zhanguo Xin; Huazhong Shi
Journal:  Plant J       Date:  2016-10-18       Impact factor: 6.417

  5 in total

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