Literature DB >> 34541141

Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader.

Magdiel Martínez1, Namyr A Martínez1, Walter I Silva1.   

Abstract

Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others ( Burgos et al., 2007 ; Martínez et al., 2016 ). Changes in intracellular calcium concentration can be measured using the calcium sensitive fluorescent ratiometric dye fura-2 AM. This method is a high throughput way to measure agonist mediated calcium responses.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Astrocyte; Calcium; Cell based analysis; Cell biology; Ion analysis; Mammalian cell line; Physiology

Year:  2017        PMID： 34541141      PMCID： PMC8413546          DOI： 10.21769/BioProtoc.2411

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  Caveolin-1 Regulates the P2Y2 Receptor Signaling in Human 1321N1 Astrocytoma Cells.

Authors:  Namyr A Martinez; Alondra M Ayala; Magdiel Martinez; Freddyson J Martinez-Rivera; Jorge D Miranda; Walter I Silva
Journal:  J Biol Chem       Date:  2016-04-18       Impact factor: 5.157

2.  P2Y2 nucleotide receptors inhibit trauma-induced death of astrocytic cells.

Authors:  Michelle Burgos; Joseph T Neary; Fernando A González
Journal:  J Neurochem       Date:  2007-09-14       Impact factor: 5.372
  2 in total
  1 in total

1.  The membrane depolarization and increase intracellular calcium level produced by silver nanoclusters are responsible for bacterial death.

Authors:  Junior Bernardo Molina-Hernandez; Luca Scotti; Clemencia Chaves-López; Antonio Aceto; Tonino Bucciarelli; Domenico Paludi; Luca Valbonetti; Katiuscia Zilli
Journal:  Sci Rep       Date:  2021-11-03       Impact factor: 4.379
  1 in total

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