Literature DB >> 34541127

[2-3H]Mannose-labeling and Analysis of N-linked Oligosaccharides.

Marina Shenkman1, Navit Ogen-Shtern1, Gerardo Z Lederkremer1.   

Abstract

Modifications of N-linked oligosaccharides of glycoproteins soon after their biosynthesis correlate to glycoprotein folding status. These alterations can be detected in a sensitive way by pulse-chase analysis of [2-3H]mannose-labeled glycoproteins, with enzymatic removal of labeled N-glycans, separation according to size by HPLC and radioactive detection in a scintillation counter.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Endoplasmic reticulum associated degradation; Glycosylation; Mannose-labeling; Mannosidase; N-linked oligosaccharide

Year:  2017        PMID： 34541127      PMCID： PMC8413558          DOI： 10.21769/BioProtoc.2393

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  11 in total

Review 1.  Protein quality control, retention, and degradation at the endoplasmic reticulum.

Authors:  Ron Benyair; Efrat Ron; Gerardo Z Lederkremer
Journal:  Int Rev Cell Mol Biol       Date:  2011       Impact factor: 6.813

2.  Membrane-bound versus secreted forms of human asialoglycoprotein receptor subunits. Role of a juxtamembrane pentapeptide.

Authors:  S Tolchinsky; M H Yuk; M Ayalon; H F Lodish; G Z Lederkremer
Journal:  J Biol Chem       Date:  1996-06-14       Impact factor: 5.157

3.  Mannosidase IA is in Quality Control Vesicles and Participates in Glycoprotein Targeting to ERAD.

Authors:  Navit Ogen-Shtern; Edward Avezov; Marina Shenkman; Ron Benyair; Gerardo Z Lederkremer
Journal:  J Mol Biol       Date:  2016-04-21       Impact factor: 5.469

Review 4.  Molecular and structural basis for N-glycan-dependent determination of glycoprotein fates in cells.

Authors:  Yukiko Kamiya; Tadashi Satoh; Koichi Kato
Journal:  Biochim Biophys Acta       Date:  2012-01-05

5.  Pulse-chase analysis of N-linked sugar chains from glycoproteins in mammalian cells.

Authors:  Edward Avezov; Efrat Ron; Yana Izenshtein; Yosef Adan; Gerardo Z Lederkremer
Journal:  J Vis Exp       Date:  2010-04-27       Impact factor: 1.355

Review 6.  N-linked sugar-regulated protein folding and quality control in the ER.

Authors:  Abla Tannous; Giorgia Brambilla Pisoni; Daniel N Hebert; Maurizio Molinari
Journal:  Semin Cell Dev Biol       Date:  2014-12-19       Impact factor: 7.727

7.  Alpha-mannosidases involved in N-glycan processing show cell specificity and distinct subcompartmentalization within the Golgi apparatus of cells in the testis and epididymis.

Authors:  S A Igdoura; A Herscovics; A Lal; K W Moremen; C R Morales; L Hermo
Journal:  Eur J Cell Biol       Date:  1999-07       Impact factor: 4.492

Review 8.  Structure and function of Class I alpha 1,2-mannosidases involved in glycoprotein synthesis and endoplasmic reticulum quality control.

Authors:  A Herscovics
Journal:  Biochimie       Date:  2001-08       Impact factor: 4.079

9.  EDEM1 accelerates the trimming of alpha1,2-linked mannose on the C branch of N-glycans.

Authors:  Nobuko Hosokawa; Linda O Tremblay; Barry Sleno; Yukiko Kamiya; Ikuo Wada; Kazuhiro Nagata; Koichi Kato; Annette Herscovics
Journal:  Glycobiology       Date:  2010-01-11       Impact factor: 4.313

10.  EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step.

Authors:  Satoshi Ninagawa; Tetsuya Okada; Yoshiki Sumitomo; Yukiko Kamiya; Koichi Kato; Satoshi Horimoto; Tokiro Ishikawa; Shunichi Takeda; Tetsushi Sakuma; Takashi Yamamoto; Kazutoshi Mori
Journal:  J Cell Biol       Date:  2014-08-04       Impact factor: 10.539
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