Literature DB >> 34541124

In vitro Demonstration and Quantification of Neutrophil Extracellular Trap Formation.

Dongsheng Jiang1, Mona Saffarzadeh2, Karin Scharffetter-Kochanek1.   

Abstract

In the recent decade, neutrophil extracellular traps (NETs) have been identified and confirmed as a new anti-microbial weapon of neutrophils. In this protocol, we describe easy methods to demonstrate NET formation by immunofluorescence staining of extracellular chromatin fiber with anti-DNA/Histone H1 antibody and quantification of NETs by using a non-cell-permeable DNA specific dye Sytox orange.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  DNA/Histone H1; NETs (neutrophil extracellular traps); Neutrophil; Sytox orange

Year:  2017        PMID： 34541124      PMCID： PMC8413513          DOI： 10.21769/BioProtoc.2386

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  11 in total

Review 1.  Integrin-mediated signaling in human neutrophil functioning.

Authors:  M A Williams; J S Solomkin
Journal:  J Leukoc Biol       Date:  1999-06       Impact factor: 4.962

2.  Neutrophil extracellular traps kill bacteria.

Authors:  Volker Brinkmann; Ulrike Reichard; Christian Goosmann; Beatrix Fauler; Yvonne Uhlemann; David S Weiss; Yvette Weinrauch; Arturo Zychlinsky
Journal:  Science       Date:  2004-03-05       Impact factor: 47.728

3.  Netting neutrophils induce endothelial damage, infiltrate tissues, and expose immunostimulatory molecules in systemic lupus erythematosus.

Authors:  Eneida Villanueva; Srilakshmi Yalavarthi; Celine C Berthier; Jeffrey B Hodgin; Ritika Khandpur; Andrew M Lin; Cory J Rubin; Wenpu Zhao; Stephen H Olsen; Matthew Klinker; David Shealy; Michael F Denny; Joel Plumas; Laurence Chaperot; Matthias Kretzler; Allen T Bruce; Mariana J Kaplan
Journal:  J Immunol       Date:  2011-05-25       Impact factor: 5.422

Review 4.  Lupus neutrophils: 'NET' gain in understanding lupus pathogenesis.

Authors:  Jason S Knight; Mariana J Kaplan
Journal:  Curr Opin Rheumatol       Date:  2012-09       Impact factor: 5.006

5.  Platelets induce neutrophil extracellular traps in transfusion-related acute lung injury.

Authors:  Axelle Caudrillier; Kai Kessenbrock; Brian M Gilliss; John X Nguyen; Marisa B Marques; Marc Monestier; Pearl Toy; Zena Werb; Mark R Looney
Journal:  J Clin Invest       Date:  2012-06-11       Impact factor: 14.808

6.  Impaired neutrophil extracellular trap (NET) formation: a novel innate immune deficiency of human neonates.

Authors:  Christian C Yost; Mark J Cody; Estelle S Harris; Nathan L Thornton; Alison M McInturff; Mark L Martinez; Nancy B Chandler; Christopher K Rodesch; Kurt H Albertine; Cathy A Petti; Andrew S Weyrich; Guy A Zimmerman
Journal:  Blood       Date:  2009-02-12       Impact factor: 22.113

7.  Neutrophil Extracellular Traps and Systemic Lupus Erythematosus.

Authors:  Yangsheng Yu; Kaihong Su
Journal:  J Clin Cell Immunol       Date:  2013-04

8.  Neutrophil extracellular traps: how to generate and visualize them.

Authors:  Volker Brinkmann; Britta Laube; Ulrike Abu Abed; Christian Goosmann; Arturo Zychlinsky
Journal:  J Vis Exp       Date:  2010-02-24       Impact factor: 1.355

9.  Neutrophil extracellular traps directly induce epithelial and endothelial cell death: a predominant role of histones.

Authors:  Mona Saffarzadeh; Christiane Juenemann; Markus A Queisser; Guenter Lochnit; Guillermo Barreto; Sebastian P Galuska; Juergen Lohmeyer; Klaus T Preissner
Journal:  PLoS One       Date:  2012-02-28       Impact factor: 3.240

10.  Characterization of rapid neutrophil extracellular trap formation and its cooperation with phagocytosis in human neutrophils.

Authors:  Mona Saffarzadeh; Hector A Cabrera-Fuentes; Florian Veit; Dongsheng Jiang; Karin Scharffetter-Kochanek; Christian Gille Gille; Suzan H M Rooijakkers; Dominik Hartl; Klaus T Preissner
Journal:  Discoveries (Craiova)       Date:  2014-06-30
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  4 in total

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Authors:  Xintong Dong; Nathachit Limjunyawong; Elizabeth I Sypek; Gaofeng Wang; Roger V Ortines; Christine Youn; Martin P Alphonse; Dustin Dikeman; Yu Wang; Mark Lay; Ruchita Kothari; Chirag Vasavda; Priyanka Pundir; Loyal Goff; Lloyd S Miller; Wuyuan Lu; Luis A Garza; Brian S Kim; Nathan K Archer; Xinzhong Dong
Journal:  Immunity       Date:  2022-07-25       Impact factor: 43.474

2.  Overexpressed angiotensin-converting enzyme in neutrophils suppresses glomerular damage in crescentic glomerulonephritis.

Authors:  Suguru Saito; Narihito Tatsumoto; Duo-Yao Cao; Nobuyuki Nosaka; Hiroshi Nishi; Daniel N Leal; Ellen Bernstein; Kenichi Shimada; Moshe Arditi; Kenneth E Bernstein; Michifumi Yamashita
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Review 3.  Citrullination in the pathology of inflammatory and autoimmune disorders: recent advances and future perspectives.

Authors:  Oskar Ciesielski; Marta Biesiekierska; Baptiste Panthu; Mirosław Soszyński; Luciano Pirola; Aneta Balcerczyk
Journal:  Cell Mol Life Sci       Date:  2022-01-25       Impact factor: 9.261

4.  Muscle Derived Mesenchymal Stem Cells Inhibit the Activity of the Free and the Neutrophil Extracellular Trap (NET)-Bond Myeloperoxidase.

Authors:  Thierry Franck; Justine Ceusters; Hélène Graide; Ange Mouithys-Mickalad; Didier Serteyn
Journal:  Cells       Date:  2021-12-10       Impact factor: 6.600
  4 in total

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