Literature DB >> 34541114

Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea.

Giovanna Salbitani1, Claudia Bottone1, Simona Carfagna1.   

Abstract

Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved spectrophotometric method to estimate the content of reduced (GSH) and oxidized (GSSG) forms of glutathione in the extremophilic microalga Galdieria phlegrea.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Glutathione; Galdieria phlegrea; Glutathione reductase; Microalgae; Redox state

Year:  2017        PMID： 34541114      PMCID： PMC8413652          DOI： 10.21769/BioProtoc.2372

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


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