Literature DB >> 34541072

Fluorescently Labelled Aerolysin (FLAER) Labelling of Candida albicans Cells.

Sneh Lata Singh1, Sneha Sudha Komath1.   

Abstract

In this protocol we describe a nonradiolabelled labelling of GPI anchor in Candida albicans. The method uses a fluorescent probe to bind specifically to GPI anchors so that the level of GPI-anchored proteins at the cell surface can be measured. The labelling does not need permeabilization of cells and can be carried out in vivo.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Candida albicans; FLAER; GPI anchor

Year:  2017        PMID： 34541072      PMCID： PMC8410404          DOI： 10.21769/BioProtoc.2303

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

Review 1.  Comprehensive analysis of glycosylphosphatidylinositol-anchored proteins in Candida albicans.

Authors:  Mathias L Richard; Armêl Plaine
Journal:  Eukaryot Cell       Date:  2006-12-22

2.  Nucleotide sequence of the gene for the hole-forming toxin aerolysin of Aeromonas hydrophila.

Authors:  S P Howard; W J Garland; M J Green; J T Buckley
Journal:  J Bacteriol       Date:  1987-06       Impact factor: 3.490

3.  Improved detection and characterization of paroxysmal nocturnal hemoglobinuria using fluorescent aerolysin.

Authors:  R A Brodsky; G L Mukhina; S Li; K L Nelson; P L Chiurazzi; J T Buckley; M J Borowitz
Journal:  Am J Clin Pathol       Date:  2000-09       Impact factor: 2.493

4.  Diagnosing PNH with FLAER and multiparameter flow cytometry.

Authors:  D Robert Sutherland; Nancy Kuek; Jeff Davidson; David Barth; Hong Chang; Erik Yeo; Sylvia Bamford; Ian Chin-Yee; Michael Keeney
Journal:  Cytometry B Clin Cytom       Date:  2007-05       Impact factor: 3.058

5.  The catalytic subunit of the first mannosyltransferase in the GPI biosynthetic pathway affects growth, cell wall integrity and hyphal morphogenesis in Candida albicans.

Authors:  Sneh Lata Singh; Ramesh Chandra Rai; Sudisht Kumar Sah; Sneha Sudha Komath
Journal:  Yeast       Date:  2016-08       Impact factor: 3.239

Review 6.  Thematic review series: lipid posttranslational modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids.

Authors:  Peter Orlean; Anant K Menon
Journal:  J Lipid Res       Date:  2007-03-14       Impact factor: 5.922

7.  Complementary adhesin function in C. albicans biofilm formation.

Authors:  Clarissa J Nobile; Heather A Schneider; Jeniel E Nett; Donald C Sheppard; Scott G Filler; David R Andes; Aaron P Mitchell
Journal:  Curr Biol       Date:  2008-07-22       Impact factor: 10.834

8.  Both mammalian PIG-M and PIG-X are required for growth of GPI14-disrupted yeast.

Authors:  Youn Uck Kim; Hisashi Ashida; Kenichiro Mori; Yusuke Maeda; Yeongjin Hong; Taroh Kinoshita
Journal:  J Biochem       Date:  2007-05-22       Impact factor: 3.387

9.  Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states.

Authors:  M W Parker; J T Buckley; J P Postma; A D Tucker; K Leonard; F Pattus; D Tsernoglou
Journal:  Nature       Date:  1994-01-20       Impact factor: 49.962
  9 in total

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