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Literature DB >> 34541067

Protein Isolation from Plasma Membrane, Digestion and Processing for Strong Cation Exchange Fractionation.

Abstract

Plasma membrane (PM) proteins play crucial roles in diverse biological processes. But their low abundance, alkalinity and hydrophobicity make their isolation a difficult task. This protocol describes an efficient method for PM proteins isolation, digestion and fractionation so that they can be well prepared for mass spectrometry analysis.

Entities: Chemical

Keywords: Fractionation; Plasma membrane proteins; RapiGest SF ; Strong cation exchange; Trypsin

Year: 2017 PMID： 34541067 PMCID： PMC8410362 DOI： 10.21769/BioProtoc.2298

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

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References

7 in total

Review 1. The application of mass spectrometry to membrane proteomics.

Authors: Christine C Wu; John R Yates
Journal: Nat Biotechnol Date: 2003-03 Impact factor: 54.908

2. Enzyme-friendly, mass spectrometry-compatible surfactant for in-solution enzymatic digestion of proteins.

Authors: Ying-Qing Yu; Martin Gilar; Peter J Lee; Edouard S P Bouvier; John C Gebler
Journal: Anal Chem Date: 2003-11-01 Impact factor: 6.986

3. Isobaric tags for relative and absolute quantification- based comparative proteomics reveals the features of plasma membrane-associated proteomes of pollen grains and pollen tubes from Lilium davidii.

Authors: Bing Han; Sixue Chen; Shaojun Dai; Ning Yang; Tai Wang
Journal: J Integr Plant Biol Date: 2010-10-22 Impact factor: 7.061

7. Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice.

Authors: Ning Yang; Tai Wang
Journal: BMC Plant Biol Date: 2017-01-05 Impact factor: 4.215

7 in total

Protein Isolation from Plasma Membrane, Digestion and Processing for Strong Cation Exchange Fractionation.

Review 1. The application of mass spectrometry to membrane proteomics.

2. Enzyme-friendly, mass spectrometry-compatible surfactant for in-solution enzymatic digestion of proteins.

3. Isobaric tags for relative and absolute quantification- based comparative proteomics reveals the features of plasma membrane-associated proteomes of pollen grains and pollen tubes from Lilium davidii.

Review 4. Less is More: Membrane Protein Digestion Beyond Urea-Trypsin Solution for Next-level Proteomics.

5. Functional differentiation of Brassica napus guard cells and mesophyll cells revealed by comparative proteomics.

6. Purification and proteomic analysis of plant plasma membranes.

7. Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice.