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Literature DB >> 34541042

Oil Red O Staining for Lipid Content in Caenorhabditis elegans.

Abstract

The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an optimized protocol for ORO staining of C. elegans and provide detailed instructions for quantifying the intensity of ORO signal in images acquired by light microscopy.

Entities: Chemical

Keywords: C. elegans; Fat; Lipid metabolism; Oil Red O

Year: 2021 PMID： 34541042 PMCID： PMC8413582 DOI： 10.21769/BioProtoc.4124

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

12 in total

1. Analyses of C. elegans fat metabolic pathways.

Authors: Alexandre Guimarães de Almeida Barros; Jason Liu; George A Lemieux; Brendan C Mullaney; Kaveh Ashrafi
Journal: Methods Cell Biol Date: 2012 Impact factor: 1.441

2. The genetics of feeding in Caenorhabditis elegans.

Authors: L Avery
Journal: Genetics Date: 1993-04 Impact factor: 4.562

3. Adipose is a conserved dosage-sensitive antiobesity gene.

Authors: Jae Myoung Suh; Daniel Zeve; Renee McKay; Jin Seo; Zack Salo; Robert Li; Michael Wang; Jonathan M Graff
Journal: Cell Metab Date: 2007-09 Impact factor: 27.287

4. C elegans: a model for exploring the genetics of fat storage.

Authors: Renée M McKay; James P McKay; Leon Avery; Jonathan M Graff
Journal: Dev Cell Date: 2003-01 Impact factor: 12.270

5. A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods.

Authors: Kelvin Yen; Thuc T Le; Ankita Bansal; Sri Devi Narasimhan; Ji-Xin Cheng; Heidi A Tissenbaum
Journal: PLoS One Date: 2010-09-16 Impact factor: 3.240

1. Dietary herbaceous mixture supplementation reduced hepatic lipid deposition and improved hepatic health status in post-peak laying hens.

Authors: Yao Zhu; Xiangli Zhang; Pengfei Du; Ziyang Wang; Pengna Luo; Yanqun Huang; Zhenhua Liu; Huaiyong Zhang; Wen Chen
Journal: Poult Sci Date: 2022-03-24 Impact factor: 4.014

1 in total

Oil Red O Staining for Lipid Content in Caenorhabditis elegans.

1. Analyses of C. elegans fat metabolic pathways.

2. The genetics of feeding in Caenorhabditis elegans.

3. Adipose is a conserved dosage-sensitive antiobesity gene.

4. C elegans: a model for exploring the genetics of fat storage.

5. A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods.

6. Lipid droplets as ubiquitous fat storage organelles in C. elegans.

7. C. elegans major fats are stored in vesicles distinct from lysosome-related organelles.

8. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O.

9. Caenorhabditis elegans as an emerging model for studying the basic biology of obesity.

Review 10. Obesity and the regulation of fat metabolism.

1. Dietary herbaceous mixture supplementation reduced hepatic lipid deposition and improved hepatic health status in post-peak laying hens.