Ensiling process for efficient biogas production from lignocellulosic substrates: Methods, mechanisms, and measures.

Ensiling has been developed as mainstream technologies to preserve lignocellulose biomass for biogas production. However, the lack of general evaluation methods and process mechanism research hinders the understanding of its effectiveness. In this context, we reviewed existing studies and proposed some key considerations: (1) For assessing the ensiling process, determined dry matter contents should be corrected according to the volatilization loss in oven-drying method to obtain accurate storage loss and methane yield; (2) For comprehensive assessments, the trade-off between storage loss and enhanced biomethane yield should be evaluated from the entire-chain process; (3) The mechanism to enhance methane yield is primarily attributed to increased lignocellulosic biodigestibility through acid-based hydrolysis and biological degradation during ensiling; (4) Measures including co-storage, increasing buffering capacity, adjusting carbon/nitrogen ratio, and additives can be adopted to increase biogas production. The proposed methods, mechanisms, and measures (3Ms) could help initiate the specific quality criteria of biogas-oriented silages.