Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides.

Literature DB >> 34536587

Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides.

Paschalis-Thomas Doulias¹, Margarita Tenopoulou², Iordanis Zakopoulos³, Harry Ischiropoulos⁴.

Abstract

Cysteine S-nitrosation mediates NO signaling and protein function under pathophysiological conditions. Herein, we provide a detailed protocol regarding the organic mercury chemoselective enrichment of S-nitrosated proteins and peptides. We discuss key aspects of the enrichment strategy and provide technical tips for the best performance of the experimental protocol.

Entities: Chemical

Keywords: Cysteine S-Nitrosation; Enrichment strategy; Nitric oxide signaling; Organic mercury; Protein; Proteomics

Mesh：

Substances：

Year: 2021 PMID： 34536587 PMCID： PMC9554525 DOI： 10.1016/j.niox.2021.09.004

Source DB: PubMed Journal: Nitric Oxide ISSN： 1089-8603 Impact factor: 4.898

Keyword Cloud
References

28 in total

1. Structural profiling of endogenous S-nitrosocysteine residues reveals unique features that accommodate diverse mechanisms for protein S-nitrosylation.

Authors: Paschalis-Thomas Doulias; Jennifer L Greene; Todd M Greco; Margarita Tenopoulou; Steve H Seeholzer; Roland L Dunbrack; Harry Ischiropoulos
Journal: Proc Natl Acad Sci U S A Date: 2010-09-13 Impact factor: 11.205

2. Gold nanoparticle enrichment method for identifying S-nitrosylation and S-glutathionylation sites in proteins.

Authors: Adam Faccenda; Christopher A Bonham; Panayiotis O Vacratsis; Xueji Zhang; Bulent Mutus
Journal: J Am Chem Soc Date: 2010-08-25 Impact factor: 15.419

3. Quantitative site-specific reactivity profiling of S-nitrosylation in mouse skeletal muscle using cysteinyl peptide enrichment coupled with mass spectrometry.

Authors: Dian Su; Anil K Shukla; Baowei Chen; Jong-Seo Kim; Ernesto Nakayasu; Yi Qu; Uma Aryal; Karl Weitz; Therese R W Clauss; Matthew E Monroe; David G Camp; Diana J Bigelow; Richard D Smith; Rohit N Kulkarni; Wei-Jun Qian
Journal: Free Radic Biol Med Date: 2012-12-28 Impact factor: 7.376

Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides.

1. Structural profiling of endogenous S-nitrosocysteine residues reveals unique features that accommodate diverse mechanisms for protein S-nitrosylation.

2. Gold nanoparticle enrichment method for identifying S-nitrosylation and S-glutathionylation sites in proteins.

3. Quantitative site-specific reactivity profiling of S-nitrosylation in mouse skeletal muscle using cysteinyl peptide enrichment coupled with mass spectrometry.

4. Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay.

Review 5. Protein S-nitrosylation: purview and parameters.

6. Protein S-Nitrosylation Controls Glycogen Synthase Kinase 3β Function Independent of Its Phosphorylation State.

7. Thioredoxin catalyzes the S-nitrosation of the caspase-3 active site cysteine.

8. Protein S-nitrosylation: a physiological signal for neuronal nitric oxide.

9. Nitric oxide regulates mitochondrial fatty acid metabolism through reversible protein S-nitrosylation.

10. Mechanism-based triarylphosphine-ester probes for capture of endogenous RSNOs.