| Literature DB >> 34534454 |
Brian E Kalmbach1, Rebecca D Hodge2, Nikolas L Jorstad2, Scott Owen2, Rebecca de Frates2, Anna Marie Yanny2, Rachel Dalley2, Matt Mallory2, Lucas T Graybuck2, Cristina Radaelli2, C Dirk Keene3, Ryder P Gwinn4, Daniel L Silbergeld5, Charles Cobbs6, Jeffrey G Ojemann7, Andrew L Ko7, Anoop P Patel8, Richard G Ellenbogen8, Trygve E Bakken2, Tanya L Daigle2, Nick Dee2, Brian R Lee2, Medea McGraw2, Philip R Nicovich2, Kimberly Smith2, Staci A Sorensen2, Bosiljka Tasic2, Hongkui Zeng2, Christof Koch2, Ed S Lein9, Jonathan T Ting10.
Abstract
In the neocortex, subcerebral axonal projections originate largely from layer 5 (L5) extratelencephalic-projecting (ET) neurons. The unique morpho-electric properties of these neurons have been mainly described in rodents, where retrograde tracers or transgenic lines can label them. Similar labeling strategies are infeasible in the human neocortex, rendering the translational relevance of findings in rodents unclear. We leveraged the recent discovery of a transcriptomically defined L5 ET neuron type to study the properties of human L5 ET neurons in neocortical brain slices derived from neurosurgeries. Patch-seq recordings, where transcriptome, physiology, and morphology were assayed from the same cell, revealed many conserved morpho-electric properties of human and rodent L5 ET neurons. Divergent properties were often subtler than differences between L5 cell types within these two species. These data suggest a conserved function of L5 ET neurons in the neocortical hierarchy but also highlight phenotypic divergence possibly related to functional specialization of human neocortex.Entities:
Keywords: cross-species; dendrite; dendritic spike; gene expression; human; intrinsic membrane properties; patch-clamp physiology; patch-seq; pyramidal neuron; transcriptomics
Mesh：
Year: 2021 PMID： 34534454 PMCID： PMC8570452 DOI： 10.1016/j.neuron.2021.08.030
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 18.688