Literature DB >> 34532548

Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro.

He Sun1, Yuanyuan Luo1, Yansong Miao1,2.   

Abstract

Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin purification and polymerization assays in this protocol, the readers could quickly get the affordable and straightforward assays to study actin dynamics.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Actin Nucleation; Actin Polymerization; Actin Purification; Formin; Pyrene Assay

Year:  2018        PMID： 34532548      PMCID： PMC8342129          DOI： 10.21769/BioProtoc.3102

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

Review 1.  Actin and Actin-Binding Proteins.

Authors:  Thomas D Pollard
Journal:  Cold Spring Harb Perspect Biol       Date:  2016-08-01       Impact factor: 10.005

Review 2.  Actin, a central player in cell shape and movement.

Authors:  Thomas D Pollard; John A Cooper
Journal:  Science       Date:  2009-11-27       Impact factor: 47.728

3.  Profilin Negatively Regulates Formin-Mediated Actin Assembly to Modulate PAMP-Triggered Plant Immunity.

Authors:  He Sun; Zhu Qiao; Khi Pin Chua; Alma Tursic; Xu Liu; Yong-Gui Gao; Yuguang Mu; Xingliang Hou; Yansong Miao
Journal:  Curr Biol       Date:  2018-05-31       Impact factor: 10.834

Review 4.  Clathrin-mediated endocytosis in budding yeast.

Authors:  Jasper Weinberg; David G Drubin
Journal:  Trends Cell Biol       Date:  2011-10-20       Impact factor: 20.808
  4 in total
  1 in total

1.  Membrane nanodomains modulate formin condensation for actin remodeling in Arabidopsis innate immune responses.

Authors:  Zhiming Ma; Yanbiao Sun; Xinlu Zhu; Liang Yang; Xu Chen; Yansong Miao
Journal:  Plant Cell       Date:  2022-01-20       Impact factor: 12.085
  1 in total

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