Literature DB >> 3453214

A non-linear regression program in BASIC for estimating Km and Vmax.

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Abstract

This paper gives a program in BASIC for calculating the kinetic parameters Km and Vmax for an enzyme reaction from a set of paired values of reaction velocity at given substrate concentrations. An initial estimate of the two parameters is made using a weighted linear regression, these values are then used in an iterative process to fit the data to the Michaelis-Menten equation and give final values of Km and Vmax with their associated standard errors.

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Year:  1987        PMID: 3453214     DOI: 10.1093/bioinformatics/3.1.49

Source DB:  PubMed          Journal:  Comput Appl Biosci        ISSN: 0266-7061


  2 in total

1.  Biochemical and immunological characterization of X-linked ichthyosis.

Authors:  X Fan; L Petruschka; K Wulff; U Grimm; F H Herrmann
Journal:  J Inherit Metab Dis       Date:  1993       Impact factor: 4.982

2.  Comparison of various estimation methods for the parameters of Michaelis-Menten equation based on in vitro elimination kinetic simulation data.

Authors:  Yong-Soon Cho; Hyeong-Seok Lim
Journal:  Transl Clin Pharmacol       Date:  2018-03-16
  2 in total

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