| Literature DB >> 3453214 |
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Abstract
This paper gives a program in BASIC for calculating the kinetic parameters Km and Vmax for an enzyme reaction from a set of paired values of reaction velocity at given substrate concentrations. An initial estimate of the two parameters is made using a weighted linear regression, these values are then used in an iterative process to fit the data to the Michaelis-Menten equation and give final values of Km and Vmax with their associated standard errors.Mesh:
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Year: 1987 PMID: 3453214 DOI: 10.1093/bioinformatics/3.1.49
Source DB: PubMed Journal: Comput Appl Biosci ISSN: 0266-7061