Pentraxin 3 levels and correlation with disease severity in patients with acute rheumatic fever.

OBJECTIVES: This study aims to investigate serum pentraxin 3 (PTX3) levels during acute episode of acute rheumatic fever (ARF) and their relationship with disease severity. PATIENTS AND METHODS: The prospective study was conducted between January 2015 and December 2018 and included 52 ARF patients (22 girls, 30 boys, mean age 10.7±2.1 years; range, 5 to 16 years) experiencing an acute episode and 22 healthy children (13 girls, 9 boys, mean age 10.3±3.8 years; range, 5 to 16 years). ARF patients were classified into three groups based on the clinical course: isolated arthritis (n=17), mild carditis (n=19), and moderate/severe carditis (n=16). Blood samples were collected from all patients before treatment and from the healthy children in the control group to measure PTX3 levels. PTX3 was measured using sandwich enzyme-linked immunosorbent assay method.
RESULTS: Plasma PTX3 levels were significantly higher in ARF group compared to the control group (4.7±5.2 and 1.2±1.7 ng/mL, p<0.001). Subgroup analysis of serum PTX3 levels in ARF patients with isolated arthritis, mild carditis, and moderate/severe carditis (3.2±3.1 ng/mL, 4.3±5 ng/mL, and 6.7±6.6 ng/mL, respectively) showed that serum PTX3 was significantly higher in the moderate/severe carditis group compared to the other groups (p<0.05). Analysis of echocardiographic data showed that serum PTX3 was positively correlated with left ventricular end-diastolic diameter, left atrial diameters, and mitral A velocity and negatively correlated with E/A ratio (p<0.05; r=0.231, 0.402, 0.562, -0.586, respectively).
CONCLUSION: High PTX3 level during an acute episode of ARF may help predict the clinical course and the severity of accompanying carditis. However, prospective studies with larger sample sizes are needed.

Introduction

Acute rheumatic fever (ARF) is a systemic inflammatory connective tissue disease and one of the nonsuppurative, delayed complications of pharyngitis or tonsillitis caused by group A beta-hemolytic streptococci.[1] Signs and symptoms affecting the joints, heart, brain, or skin arise due to abnormal immune response about three weeks after a typical throat infection.[2] The main cause of morbidity and mortality from the disease is carditis; in the acute period, it can lead to heart failure and death due to pancarditis.[3] There are no specific biomarkers for the diagnosis of ARF, and evaluating disease activity at presentation is difficult.

Long pentraxin 3 (PTX3) is a member of pentraxin family produced by stromal and myeloid cells in response to proinflammatory signals and microbial agents.[4] PTX3 is important in the clearance of apoptotic cells, modulation of inflammation and angiogenesis, and formation of extracellular matrix. It belongs to the same protein family as C-reactive protein (CRP); however, while CRP is mainly synthesized in the liver, PTX3 is mostly produced locally in the area of inflammation.[5,6] PTX3 has been associated with inflammation in cardiovascular diseases such as heart failure, atherosclerosis, acute coronary syndrome, peripheral vascular diseases, and rheumatic mitral valve stenosis.[4,7-9] To our knowledge, there are no previous studies in the literature regarding serum PTX3 levels in ARF patients. In this study, we aimed to investigate serum PTX3 levels during acute episode of ARF and their relationship with disease severity.

Patients and Methods

This prospective study was conducted in the Pediatric Cardiology Department of Pamukkale University Faculty of Medicine between January 2015 and December 2018. The study group consisted of 52 patients (22 girls, 30 boys, mean age 10.7±2.1 years; range, 5 to 16 years) with acute ARF episode. ARF was diagnosed based on the modified Jones criteria. The 2002 version of the modified Jones criteria[10] was used until the year 2016, while the 2015 updated version[11] was used after the year 2016. Patients with recurrent ARF were excluded. Valve involvement in ARF patients was evaluated based on the 2012 World Heart Federation Rheumatic Carditis criteria.[12] ARF patients were classified into three groups based on the clinical course of the acute episode: isolated arthritis (n=17), mild carditis (n=19), and moderate/severe carditis (n=16).

The control group consisted of 22 voluntary, age- and sex-matched healthy children (13 girls, 9 boys, mean age 10.3±3.8 years; range, 5 to 16 years) with no history of hypertension, renal or cardiac diseases. Children in the control group had normal physical examination, biochemical analysis and echocardiographic evaluation. Children who had received nonsteroidal anti-inflammatory drugs were excluded. There were no other systemic or cardiovascular diseases that could affect serum PTX3 level. The study protocol was approved by the Pamukkale University Faculty of Medicine Ethics Committee (Approval No: 60116787-020/53183). A written informed consent was obtained from parents of all patients and control subjects. The study was conducted in accordance with the principles of the Declaration of Helsinki.

Blood samples were collected from the children diagnosed with ARF before treatment and from the healthy children in the control group to measure PTX3 levels. After centrifugation, samples were stored at -80°C. Serum PTX3 levels were measured using a sandwich enzyme-linked immunosorbent assay kit (Bioassay Technology, Shanghai, China, Catalog no: E1938Hu). Absorbance values at a wavelength of 450 nm were measured using a BioTek-Elx800 Absorbance Microplate Reader (BioTek Instruments Inc., Winooski, VT, USA) and PTX3 concentrations were calculated. Sensitivity of the assay for PTX3 is <0.05 ng/mL.

All ARF patients and control subjects underwent two-dimensional, motion-mode (M-mode), and Doppler examination with GE Vingmed Vivid 7 echocardiography (GE Vingmed, Ultrasound AS, Horten, Norway) and multifrequency transducer (2.5-4 MHz). Images were acquired without sedation, with children in left lateral decubitus position. The mean of three consecutive measurements was recorded. M-mode measurements were performed at the level of the tips of the mitral valve leaflets in the parasternal long-axis view of the left ventricle (LV).[13] Standard parameters measured in parasternal long-axis by M-mode echocardiography included left ventricular end- diastolic diameter (LVEDD), interventricular septum thickness at end-diastole (IVSTd), and left ventricular posterior wall thickness at end-diastole (LVPWTd). Left ventricular ejection and shortening fractions (LVEF, LVSF) were calculated as described previously.[14] Pulsed wave Doppler blood velocities were measured for the mitral valve using apical four-chamber view (peak E and A wave) and E/A ratio was determined.

Statistical analysis

Statistical analysis was performed using the IBM SPSS version 25.0 software (IBM Corp., Armonk, NY, USA). Continuous variables were presented as mean ± standard deviation, median (interquartile range) and qualitative variables as number (percentage). Kolmogorov-Smirnov and Shapiro-Wilk tests were used for determination of normal distribution. For independent groups comparisons, we used independent-samples t-test and one-way analysis of variance (post hoc: Tukey test) when parametric test assumptions were provided, and Mann-Whitney U test and Kruskal-Wallis variance analysis (post hoc: Mann-Whitney U test with Bonferroni correction) were used when parametric test assumptions were not provided. Differences between categorical variables were established using Chi-square test. Relationships between variables were evaluated using Spearman correlation coefficient. Statistical significance was set at p<0.05.

Results

There was no statistical difference between ARF and control groups in terms of age, sex, weight, height, body mass index (BMI), heart rate, or blood pressure (p>0.05). At admission, the ARF patients had significantly higher white blood cell (WBC) count, platelet count, CRP, erythrocyte sedimentation rate (ESR), fibrinogen, antistreptolysin O (ASO) levels and significantly lower hemoglobin level (p<0.05). Mean serum PTX3 concentration was 4.7±5.2 (range, 0.2 to 18.9) ng/mL in the ARF group and 1.2±1.7 (range, 0.1 to 5.8) ng/mL in the control group (p<0.001). The demographic characteristics and laboratory data of the ARF and control groups are shown in Table 1.

The acute episode manifested with isolated arthritis in 17 of the ARF patients (13 boys, 4 girls; mean age 10.5±1.5 years) and was accompanied by carditis in 35 ARF patients (17 boys, 18 girls; mean age 10.8±2.4 years). There was no statistical difference between the isolated arthritis, carditis, and control groups in terms of age, sex, weight, height, BMI, heart rate, or blood pressure (p>0.05). WBC and platelet counts, ESR, and CRP, fibrinogen, and ASO levels were higher in both the arthritis and carditis subgroups compared to the control group (p<0.001). Hemoglobin level was significantly lower in the carditis group compared to the control group (p<0.05). Mean serum PTX3 levels were 3.2±3.1 (range, 0.3 to 10.1) ng/mL in patients with isolated arthritis and 5.4±5.9 (range, 0.16 to 18.9) ng/mL in patients with carditis. There was a statistically significant difference in PTX3 level between the arthritis, carditis, and control groups (p<0.001). Serum PTX3 level was higher in the carditis group than the other groups, while the difference between the carditis and control groups was statistically significant (p<0.05) (Table 2).

Of 35 ARF patients with carditis, 10 (28.5%) had mitral valve insufficiency alone, two (6%) had aortic valve insufficiency alone, and 23 (65.5%) had both aortic and mitral valve insufficiency. None of the patients had mitral valve stenosis. Nineteen (54%) patients had mild carditis and 16 (46%) had moderate/severe carditis. There were no statistically significant differences between the arthritis, mild carditis, moderate/severe carditis, and control groups in terms of age, sex, weight, height, BMI, heart rate, or blood pressure (p>0.05) (Table 3). Platelet count, ESR, and CRP, fibrinogen, and ASO levels were significantly higher in the arthritis, mild carditis, and moderate/severe carditis group compared to the control group (p<0.001). WBC count was higher and hemoglobin level was lower in the mild carditis and moderate/severe carditis groups than the control group (p<0.05). The mean serum PTX3 level was 1.2±1.7 (range, 0.1 to 5.8) ng/mL in the control group, 3.2±3.1 (range, 0.3 to 10.1) ng/mL in the isolated arthritis group, 4.3±5 (range, 0.2 to 15.4) ng/dL in the mild carditis group, and 6.7±6.6 (range, 0.2 to 18.9) ng/dL in the moderate/severe carditis group. Although serum PTX3 levels were higher in all patient groups when compared with the control group, the differences were significant only in the mild and moderate/severe carditis groups (p<0.05) (Table 3) (Figure 1).

In echocardiographic analysis, no difference was detected between the groups in terms of LVPWTd, IVSTd, LVEF, or LVSF values (p>0.05). LVEDD and left atrial diameters (LAD) were significantly higher in the moderate/severe carditis group compared to all other groups (p<0.001). No difference was detected between the groups in terms of mitral valve E-wave velocity (p>0.05), while mitral valve A-wave velocity was significantly higher in the moderate/severe carditis group than in the control group (p<0.05) and E/A ratio was significantly lower in the moderate/severe carditis group compared to the other groups (p<0.05) (Table 3).

The CRP level was positively correlated with WBC and platelet count, ESR, and fibrinogen level, and negatively correlated with hemoglobin level (p<0.05; r values: 0.449, 0.265, 0.691, 0.675, -0.384, respectively). CRP level was also positively correlated with LVEDD and LAD (p<0.05; r values: 0.355, 0.263, respectively).

Serum PTX3 level was positively correlated with platelet count, ESR, and fibrinogen level and negatively correlated with hemoglobin level (p<0.05, r values: 0.416, 0.338, 0.324, -0.238, respectively). Analysis of echocardiographic data showed that PTX3 level was positively correlated with LVEDD, LAD, and mitral valve A-wave velocity and negatively correlated with E/A ratio (p<0.05; r values: 0.231, 0.402, 0.562, -0.586, respectively). The correlations between serum PTX3 levels and LVEDD, LAD, mitral A-wave velocity, and E/A are visualized in Figures 2, 3, 4, and 5.

Discussion

Acute rheumatic fever is an autoimmune disease caused by the abnormal immune response against streptococcus infections in genetically predisposed children. Inflammation in ARF involves a series of complicated factors including the cellular and humoral immune systems.[15] There are no specific biomarkers for ARF diagnosis and follow-up; the diagnosis is established clinically based on the Jones criteria.[11] This has prompted research into more specific biomarkers that clinicians can use at the time of diagnosis to determine disease severity and predict cardiac involvement or prognosis. PTX3 is a new-generation acute phase reactant that is structurally related to CRP and serum amyloid P, and its role in the inflammatory process is still under investigation.[16] Our study is important because it is the first in the literature to evaluate serum PTX3 levels in ARF patients during an acute episode. The serum PTX3 levels were found to be increased in ARF patients when compared to healthy children in our study. In addition, the PTX3 levels were higher in mild and moderate/severe carditis groups.

The two most important characteristics of PTX3 are that it is locally produced in the endothelium and it reaches peak levels faster than CRP.[6,17,18] It is reported that PTX3 level is elevated in cardiovascular diseases and can be regarded as a biomarker of cardiovascular disease in the general population.[18] High serum PTX3 levels have also been associated with increased cardiovascular complications in conditions such as Cushing’s and Behçet’s diseases and in hemodialysis patients.[19-21] In addition, there are studies on its potential superiority to CRP as a biomarker of cardiovascular disease due to its rapid response and ability to reflect vascular inflammation.[9,22,23] In a study conducted with childhood-onset systemic lupus erythematosus patients, it was found that serum PTX3 levels significantly increased in the presence of vasculitis, and it was reported that serum PTX3 levels could be useful in detecting subclinical vascular involvement.[24] In another study, serum PTX3 levels were found to be higher in patients with juvenile scleroderma than healthy controls, but there was no correlation with vascular complications. However, a positive correlation was found between serum PTX3 level and skin thickening and fibrosis.[25] In our study, CRP levels were higher in all ARF subgroups (arthritis and carditis) compared to the control group, while PTX3 level was only significantly higher in patients with carditis compared to healthy controls. These findings support the view that PTX3 may be a more specific biomarker than CRP in vascular inflammation and that high serum PTX3 levels may predict carditis in ARF patients. In the future steps of our study, evaluating the relationship between serum PTX3 levels during an acute episode and rheumatic heart disease, which is a chronic complication of the ARF, may be a guide in explaining the relationship between PTX3 and fibrosis development.

Studies on PTX3-deficient mouse models demonstrated greater ischemia/reperfusion- induced cardiac damage after myocardial infarction and increased atherosclerotic lesions with a high- fat diet. Therefore, PTX3 is believed to have cardioprotective and atheroprotective effects.[26,27] On the other hand, Swada et al.[28] reported that although patients with abdominal aortic aneurysm showed no difference in serum PTX3 level, tissue PTX3 expression was increased compared with the control group and was negatively correlated with maximum aorta diameter. We detected no correlation between PTX3 and carditis severity in the present study. However, similar to the study by Swada et al.,[28] future studies evaluating tissue expression of PTX3 in addition to serum PTX3 levels will improve our understanding of the role of PTX3 in the pathogenesis of ARF and its relationship with carditis.

Mitral valve involvement is observed most frequently in ARF, followed by aortic valve involvement.[29] This involvement usually manifests as valve insufficiency during the initial episode. Left side valve insufficiency causes increased volume load and diameter in the left atrium and LV. In our study, left ventricular end-diastolic and LAD were greater in the moderate/severe carditis group than in the other groups due to valve involvement. While no difference was detected between the groups in terms of left ventricular systolic functions, we found that left ventricular late diastolic wave velocity was higher and E/A ratio was lower in the moderate/severe carditis group compared to the other groups. Our results indicate that left ventricular diastolic functions are affected in the moderate/severe carditis group. In their study on individuals with Behçet’s disease, Çalık et al.,[21] reported a correlation between serum PTX3 level and LAD and mitral E/A ratio. Similarly, in the present study, serum PTX3 level was positively correlated with left ventricular end-diastolic and LAD and mitral late diastolic pulse wave, and negatively correlated with E/A ratio in patients with acute episode of ARF. Studies evaluating echocardiographic data together with serum PTX3 data in larger series may shed light on the role of PTX3 in the pathophysiology of carditis.

This study has two limitations that should be acknowledged. Firstly, our study included a small sample size. Secondly, only serum PTX3 levels were analyzed, and tissue PTX3 expression could not be shown. Additionally, we were not able to evaluate the role of serum PTX3 level in the prediction of permanent valve damage in patients under follow-up.

In conclusion, our study is the first to demonstrate elevated serum PTX3 levels in patients during an acute episode of ARF. High serum PTX3 levels may help predict the development of carditis during the course of an acute ARF episode. However, further prospective studies that include larger series are needed.

Table 1

Demographic characteristics and laboratory data of ARF patients and healthy subjects

	ARF group (n=52)					Control group (n=22)
	n	Mean±SD	Min-Max	Median	IQR	n	Mean±SD	Min-Max	Median	IQR	p
Age (year)		10.7±2.1	5-16	11	9-12		10.3±3.8	5-16	10,5	6,7-14	>0.05’
Sex											>0.05"
Male	30					9
Female	22					13
Weight (kg)		41.5±12.8	20-71	41.5	31-51.5		37.9±17.4	18-70	31	22.7-54.2	>0.05’
Height (cm)		144.9±13	112-169	145.5	135.2-156		140±22.6	107-175	139	118.7-163.5	>0.05***
BMI (kg/m=)		19.3±3.9	12.3-28.1	19.6	16-21		18.2±3.3	14.3-24.8	17.4	15.7-19.4	>0.05*
Heart rate (beats/min)		81.4±4.7	75-100	80	78-84		80.8±5.2	72-95	80	78-84	>0.05*
Systolic blood pressure		108.4±10.6	89-130	110	100-115		105.8±9.9	90-125	107	95.7-115	>0.05*
Diastolic blood pressure		65.2±9.1	47-90	65	60-75		65.2±9.1	50-85	65	58.7-72.5	>0.05*
WBC count (/mm3)		11,353±3,327	6,610-22,400	10,870	8,615-13,262		7,906±l,813	3,370-10,080	8,325	6,895-9,290	0.000*
Hemoglobin (g/dL)		11.6±1	9.1-14	11.6	11-12.3		12.4±0.7	11.4-14.1	12.2	11.8-12.9	0.003***
Platelet count (/mm3)		422,000±133,330	166,000-705,000	412,500	325,750-530,000		284,955±45,034	210,000-380,000	280,000	247,000-330,000	0.000***
CRP (mg/dL)		8.8±6.6	2-27	6.8	3-12.8		0.14±0.14	0.03-0.5	0.06	0.05-0.2	0.000*
ESR (mm/h)		80.4±22.6	36-130	76	62.2-99.5		14.3±4.4	8-22	14	10-18	0.000***
Fibrinogen (mg/dL)		662.6±352	249-2918	639.5	500.7-752.2		234.6±23	188-286	238	220-248	0.000*
ASO (lU/mL)		968.9±630.4	113-3862	893	557-1150		60±38.6	4-122	54	21.5-99.5	0.000*
Pentraxin 3 (ng/mL)		4.7±5.2	0.16-18.9	2.78	0.50-7.25		1.2±1.7	0.1-5.8	0.41	0.1-1.15	0.000*
ARF: Acute rheumatic fever; SD: Standard deviation; Min: Minimum; Max: Maximum; IQR: Interquartile range; BMI: Body mass index; WBC: White blood cell; CRP: C-reactive protein; ESR: Erythrocyte sedimentation rate; ASO: Antistreptolysin O; * Mann-Whitney U test; ** Chi-square test; *** Independent-samples t-test.

Table 2

Demographic characteristics and laboratory results of arthritis, carditis, and control groups

	Isolated arthritis (n=17) (Group 1)					Carditis (n=35) (Group 2+3)					Control (n=22) (Group 4)
	n	Mean±SD	Min-Max	Median	IQR	n	Mean±SD	Min-Max	Median	IQR	n	Mean±SD	Min-Max	Median	IQR	p	Significant group comparisons
Age (year)		10.5±1.5	8-13	11	9-12		10.8±2.4		11	9-12		10.3±3.8		10.5	6.7-14	>0.05†
Sex																>0.05**
Male	13					17					9
Female	4					18					13
Weight (kg)		37.6±10	21-57	36	30-46		43.4±13.7	20-71	44	31.2-53		37.9±17.4	18-70	31	22.7-54.2	>0.05‡
Height (cm)		144.3±9.9	129-162	142	135.5-152		145.2±14.4	112-169	147	135-157		140±22.6	107-175	139	118.7-163.5	>0.05†
BMI (kg/m2)		17.8±3.5	12.3-25.3	17.3	15.3-20.7		20±3.9	12.8-28.1	20.5	16.6-23.8		18.2±3.3	14.3-24.8	17.4	15.7-19.4	>0.05‡
Heart rate (beats/min)		81.8±3.1	76-86	82	79-84.5		81.2±5.3	75-100	80	78-84		80.8±5.2	72-95	80	78-84	>0.05†
Systolic blood pressure		106.9±10.7	89-130	105	100-110		109.2±10.6	90-130	110	100-115		105.8±9.9	90-125	107	95.7-115	>0.05†
Diastolic blood pressure		64.8±9.4	47-80	62	60-72.5		68.2±10.1	50-90	68	60-78		65.2±9.1	50-85	65	58.7-72.5	>0.05†
WBC count (/mm3)		10,663±3,727	6,610-22,400	9,740	7,955-11,620		11,688±3,117	6,880-18,820	11,080	8,840-13,500		7,906±1,813	3,370-10,080	8,325	6,895-9,290	0.000‡	1-4, 2+3-1
Hemoglobin (g/dL)		12±0.9	10.5-13.7	11.9	11.4-12.65		11.5±1	9.1-14	11.25	10.7-12.2		12.4±0.7	11.4-14.1	12.2	11.8-12.9	0.002†	2+3-4
Platelet count (/mm3)		422,706±136,612	177,000-701,000	413,000	348,000-516,000		421657±133.726	166,000-705,000	412,000	312,000-548,000		284.955±45.034	210,000-380,000	280,000	247,000-330,000	0.000†	1-4, 2+3-4
CRP (mg/dL)		8.2±6.3	2.4-24	5.75	3-12.9		9±6.8	2-17	7	3.1-12.8		0.14±0.14	0.03-0.5	0.06	0.05-0.2	0.000‡	1-4, 2+3-4
ESR (mm/h)		75.5±21.4	36-115	75.5	60.5- 91.5		82.8±23.1	39-130	78	64-100		14.3±4.4	8-22	14	10-18	0.000†	1-4, 2+3-4
Fibrinogen (mg/dL)		577.6±163.9	340-826	580	443-755.5		703.9±409.6	249-2918	650	567-750		234.6±23	188-286	238	220-248	0.000‡	1-4, 2+3-4
ASO (IU/mL)		959±548.2	243-2208	780	546.5-1398		973.7±674.3	113-3862	907	575-1129		60±38.6	4-122	54	21.5-99.5	0.000‡	1-4, 2+3-4
Pentraxin 3 (ng/mL)		3.2±3.1	0.26-10.08	2.3	0.35-5.17		5.4±5.9	0.16-18.9	3.05	0.5-10.2		1.2±1.7	0.1-5.8	0.41	0.1-1.15	0.000‡	2+3-4
ARF: Acute rheumatic fever; SD: Standard deviation; Min: Minimum; Max: Maximum; IQR: Interquartile range; BMI: Body mass index; WBC: White blood cell; CRP: C-reactive protein; ESR: Erythrocyte sedimentation rate; ASO: Antistreptolysin O; * Chi-square test; † One-way analysis of variance test; ‡ Kruskal- Wallis test.

Table 3

Demographic characteristics and laboratory results of arthritis, mild carditis, moderate/severe carditis, and control groups

	Isolated arthritis (n=17) (Group 1)		Mild carditis (n=19) (Group 2)		Moderate/severe carditis (n=16) (Group 3)		Control (n=22) (Group 4)		p	Significant group comparisons
	Mean±SD (Min-Max)	Median (IQR)	Mean±SD (Min-Max)	Median (IQR)	Mean±SD (Min-Max)	Median (IQR)	Mean±SD (Min-Max)	Median (IQR)
Age (year)	10.5±1.5 (8-13)	11 (9-12)	10.6±2.4 (5-14)	11 (9-12)	11±2.4(6-16)	11 (10-12)	10.3±3.8 (5-16)	10.5(6.7-14)	0.883†
Sex (n) Male Female		134		910	88		913		0.148*
Weight (kg)	37.6±10(21-57)	36 (30-46)	44.6±13.6(20-71)	45.6 (34-55)	41.8±14(20-71)	42.5(31-52.25)	37.9±17.4(18-70)	31(22.7-54.2)	0.369‡
Height (cm)	144.3±9.9(129-162)	142(135.5-152)	147.5±13.7(112-164)	153(137-157)	142.5±15.1(115-169)	140(130.5-153)	140±22.6(107-175)	139(118.7-163.5)	0.547‡
BMI (kg/m2)	17.8±3.5(12.3-25.3)	17.3(15.3-20.7)	20.1±4(12.8-28)	20.7(15.9-23.7)	20±3.9(14.8-28.2)	19.69(16.9-23.3)	18.2±3.3(14.3-24.8)	17.4(15.7-19.4)	0.146‡
Heart rate (beats/min)	81.8±3.1(76-86)	82(79-84.5)	79.4±3.7(75-88)	78 (78-80)	83.2±6.2(78-100)	80(78.5 -88)	80.8±5.2(72-95)	80 (78-84)	0.125‡
Systolic blood pressure	106.9±10.7(89-130)	105 (100-110)	106.8±10(89-130)	110 (100-115)	109±ll(90-130)	112(106-115.7)	105.8±9.9(90-125)	107(95.7-115)	0.691‡
Diastolic blood pressure	64.8±9.4(47-80)	62(60-72.5)	67.2±10.1(50-80)	70 (60-78)	69.3±10.2(55-90)	66.5(60.7-77.2)	65.2±9.1(50-85)	65(58.7-72.5)	0.510†
WBC count (/mm3)	10663±3727(6610-22400)	9740(7955-11620)	11335±3047(6880-18000)	10940(8500-13270)	12107±3247(7840-18820)	11105(9917-14550)	7906±1813(3370-10080)	8325(6895-9290)	0.000‡	2-4, 3-4
Hemoglobin (g/dL)	12±0.9(10.5-13.7)	11.9 (11.4(12.65)	11.7±0.8(10.3-13.3)	11.57(11-12.2)	11.3±1.2(9.1-14)	11.1(10.6-12.2)	12.4±0.7(11.4-14.1)	12.2(11.8-12.9)	0.004†	2-4, 3-4
Platelet count (/mm3)	422706±136612(177000-701000)	413000(348000-516000)	394158±136485(209000-705000)	355000(285000-488000)	454313±136485(166000-657000)	433000(392750-548750)	284955±45034(210000-380000)	280000(247000-330000)	0.000†	1-4, 2-4,3-4
CRP (mg/dL)	8.2±6.3(2.4-24)	5.75(3-12.9)	9±6.5(2.8-27)	7(3.5-11.7)	9±7.3(2-25)	6.3(2.5-13.6)	0.14±0.14(0.03-0.5)	0.06(0.05-0.2)	0.000‡	1-4, 2-4,3-4
ESR (mm/h)	75.5±21.4(36-115)	75.5 (60.5- 91.5)	80.8±22.4(39-119)	84 (58-100)	85.1±24.4(51-130)	76(68-106.75)	14.3±4.4 (8-22)	14 (10-18)	0.000†	1-4, 2-4,3-4
Fibrinogen (mg/dL)	577.6±163.9(340-826)	580(443-755.5)	621.5±123.3(410-884)	639(540-700)	801.7±586(249-2918)	729(586.7-778-7)	234.6±23(188-286)	238(220-248)	0.000‡	1-4, 2-4,3-4
ASO (lU/mL)	959±548.2(243-2208)	780(546.5-1398)	1109.5±792(249-3862)	937 (792-1157)	812.5±476.4(113-1983)	864(443.7-961.7)	60±38.6(4-122)	54(21.5-99.5)	0.000‡	1-4, 2-4,3-4
Pentraxin 3 (ng/mL)	3.2±3.1(0.26-10.08)	2.3(0.35-5.17)	4.3±5(0.16-15.4)	1.6(0.5-7.4)	6.7±6.6(0.23-18.9)	3.87(0.7-13.2)	1.2±1.7(0.1-5.8)	0.41(0.1-1.15)	0.002‡	2-4, 3-4
LVPWTd (cm)	0.81±0.1(0.6-1)	0.8(0.7-0.9)	0.82±0.15(0.6-1)	0.8(0.7-1)	0.82±0.11(0.7-1)	0.8(0.7-0.9)	0.74±0.12(0.6-1)	0.7(0.6-0.8)	>0.05‡
LVEDD (cm)	4.14±0.43 (3.5-4.9)	4(3.8-4.5)	4.25±0.46 (3.3-5.3)	4.2(4-4.5)	4.67±0.51 (4.1-5.5)	4.5(4.2-5.2)	3.9±0.45(3.3-4.7)	3.8(3.6-4.4)	0.000‡	1-3, 2-3,3-4
IVSTd (cm)	0.82±0.1(0.6-1)	0.8(0.75-0.9)	0.78±0.12(0.6-1)	0.8 (0.7- 0.9)	0.80±0.11(0.6-1)	0.8(0.7-0.9)	0.79±0.11(0.6-1)	0.75(0.7-0.9)	>0.05‡
LAD (cm)	2.85±0.29 (2.3-3.3)	2.8(2.6-3.1)	3 ±0.4(2.3-3.6)	3(2.8-3.4)	3.4±0.38(2.8-4)	3.35(3-3.7)	2.67±0.36(2-3.3)	2.65(2.4-3)	0.000†	1-3, 2-3, 2-4, 3-4
LVEF (%)	80.6±4.1(72-86)	81.3(77-84.6)	79±5.3 (69-86)	79.5(75.6-83.4)	76.7±4.7(64-83)	78.1(73.4-78.4)	80.7±3.8(72-88)	80.6(77.8-81.8)	>0.05†
LVSF (%)	42.4±4.1(34.8-48.9)	42.8(38.6-46.4)	40.9±5(32.4-48.9)	41(37.5-45)	38.7±4(29.2-45.2)	39.76(35.7-40)	42.4±3.8(34.7-51)	42(39.4-43.2)	>0.05†
Mitral E (m/s)	99.4±10.5(82-118)	101(89-108.5)	96.6±9.4(78-110)	98(91-104)	96.8±9.1(76-112)	96.5(92.2-104)	98.1±6.6(88-110)	98(94-103.5)	>0.05†
Mitral A (m/s)	48.6±7.6(38-62)	48(42.5-54.5)	49.1±10.1(34-67)	49 (40-59)	55.9±10.5(41-74)	53.5(46.5-67)	46.5±5.5 (40-58)	45.5(41.7-50.5)	0.011†	3-4
E/A ratio	2 ±0.22(1.51-2.42)	2.1(1.96-2.22)	2±0.29(1.6-2.54)	2(1.72-2.27)	1.77±0.24(1.37-2.24)	1.75(1.55-1.93)	2.13±0.25(1.69-2.58)	2.16(1.9-2.4)	0.003†	1-3, 2-3,3-4
SD: Standard deviation; Min: Minimum; Max: Maximum; IQR: Interquartile range; BMI: Body mass index; WBC: White blood cell; CRP: C-reactive protein; ESR: Erythrocyte sedimentation rate; ASO: Antistreptolysin O; LVPWTd: Left ventricular posterior wall thickness at diastole; LVEDD: Left ventricular end-diastolic diameter; IVSTd: Interventricular septum thickness at diastole; LAD: Left atrium diameter; LVEF: Left ventricular ejection fraction; LVSF: Left ventricular shortening fractions; Mitral E: Mitral early diastolic flow rate; Mitral A: Mitral late diastolic flow rate; * Chi-square test; † One-way analysis of variance test; ‡ Kruskal-Wallis test.