Antimicrobial electrospun nanofiber mats of NaOH-hydrolyzed chitosan (HCS)/PVP/PVA incorporated with in-situ synthesized AgNPs: Fabrication, characterization, and antibacterial activity.

This work aims to improve the electrospinability and antibacterial activity of chitosan (CS) - based nanofibers. Three approaches consisting of reducing molecular weight of CS by NaOH hydrolysis (HCS), blending with two carrying polymers (polyvinylpyrrolidone (PVP) and polyvinyl alcohol (PVA)) and incorporating with in-situ synthesized silver nanoparticles (AgNPs) were integrated simultaneously for the first time to fabricate the HCS-AgNPs/PVP/PVA multicomponent nanofibers. The electrospinning parameters were optimized to obtain the smooth and uniform nanofibers without beads of both HCS/PVP/PVA and HCS-AgNPs/PVP/PVA systems. The presence of in-situ AgNPs in the multicomponent blends gives the better electrospinning performance and the lowest fiber diameter of 139 nm. In addition, the thermal properties, thermal stability and crystallinity index of both nanofibers also increased with increasing HCS or HCS-AgNPs fractions. Finally, the best antibacterial activity of HCS/PVP/PVA and HCS-AgNPs/PVP/PVA nanofibers against E. coli was found to be 74.4% and 99.9%, respectively. The significant enhancement in bactericidal activity of HCS-AgNPs/PVP/PVA nanofibers against E. coli is due to the synergistic properties of HCS/PVP/PVA blends and AgNPs. Both nanofiber mats displayed the excellent structural stability in moisture environment for at least 7 days. Therefore, the HCS-AgNPs/PVP/PVA nanofibers could be a potential material for applying in the medical purpose.