Arzu Ay1, Tevfik Gulyasar2, Nevra Alkanli3, Tammam Sipahi2, Irfan Cicin4, Zafer Kocak5, Necdet Sut6. 1. Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey. arzuay@trakya.edu.tr. 2. Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey. 3. Department of Biophysics, Faculty of Medicine, Haliç University, Istanbul, Turkey. nevraalkanli@halic.edu.tr. 4. Department of Medical Oncology, Faculty of Medicine, Trakya University, Edirne, Turkey. 5. Department of Radiation Oncology, Faculty of Medicine, Trakya University, Edirne, Turkey. 6. Department of Biostatistics and Medical Informatics, Faculty of Medicine, Trakya University, Edirne, Turkey.
Abstract
BACKGROUND: The aim of this study is to investigate of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patient with colorectal cancer. Mateials and Methods. Genotype distributions of GSTM1 gene variations were determined using real-time polymerase chain reaction method. Serum trace element levels were determined using atomic absorption spectrophotometer method and plasma MDA levels were measurement by spectrophotometric method. RESULTS: Serum Cu levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GA heterozygous genotype of the GSTM1 (rs 112,778,559) gene variation compared to healthy controls (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in patients carrying GG homozygous genotype of the GSTM1 (rs 112778559) gene variation compared to healthy controls carrying same genotype (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GG homozygous genotype of the GSTM1 (rs 12068997) gene variation compared to healthy controls (p < 0.05). On the other hand, serum Se levels were detected significantly lower in CRC patients carrying GA heterozygous and GG homozygous genotypes for GSTM1 (rs 112,778,559) and (rs 12,068,997) gene variations compared to healthy controls (p < 0.05). CONCLUSION: In our study, the evaluation of serum Cu, Zn and Se trace element levels and plasma MDA levels according to GSTM1 gene variations genotype distributions were enabled to obtain important biomarkers in terms of CRC development and progression.
BACKGROUND: The aim of this study is to investigate of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patient with colorectal cancer. Mateials and Methods. Genotype distributions of GSTM1 gene variations were determined using real-time polymerase chain reaction method. Serum trace element levels were determined using atomic absorption spectrophotometer method and plasma MDA levels were measurement by spectrophotometric method. RESULTS: Serum Cu levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GA heterozygous genotype of the GSTM1 (rs 112,778,559) gene variation compared to healthy controls (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in patients carrying GG homozygous genotype of the GSTM1 (rs 112778559) gene variation compared to healthy controls carrying same genotype (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GG homozygous genotype of the GSTM1 (rs 12068997) gene variation compared to healthy controls (p < 0.05). On the other hand, serum Se levels were detected significantly lower in CRC patients carrying GA heterozygous and GG homozygous genotypes for GSTM1 (rs 112,778,559) and (rs 12,068,997) gene variations compared to healthy controls (p < 0.05). CONCLUSION: In our study, the evaluation of serum Cu, Zn and Se trace element levels and plasma MDA levels according to GSTM1 gene variations genotype distributions were enabled to obtain important biomarkers in terms of CRC development and progression.
Authors: Mehmet Kaba; Necip Pirinççi; Mehmet Bilgehan Yüksel; İlhan Gecit; Mustafa Güneş; Murat Demir; HurremTuran Akkoyun; Halit Demir Journal: Int Braz J Urol Date: 2015 Nov-Dec Impact factor: 1.541
Authors: Justyna Klusek; Anna Nasierowska-Guttmejer; Artur Kowalik; Iwona Wawrzycka; Piotr Lewitowicz; Magdalena Chrapek; Stanisław Głuszek Journal: Oncotarget Date: 2018-04-20