| Literature DB >> 34495509 |
Jonatha Dos Santos Silva1, Layanna Rebouças de Santana Cerqueira1, Wayne Bextine Hunter2, Eduardo Chumbinho de Andrade3.
Abstract
RNA interference (RNAi) comprises a natural mechanism of gene regulation and antiviral defense system in eukaryotic cells, and results in sequence-specific degradation of RNAs. Recent scientific studies demonstrate the feasibility of use RNAi-based strategies to control pest and pathogens in plants. A key step in developing RNAi-based products is a reliable method to appropriated screening of selected dsRNAs.Herein presented are a bioassay for screening dsRNAs to control the Asian citrus psyllid (ACP), Diaphorina citri, vector of citrus Huanglongbing (HLB) and other hemipterans. The RNAi feeding bioassay, called in plant system (iPS), uses vegetative new growth citrus flush to deliver double-strand RNA (dsRNA ) to ACP during natural feeding .Entities:
Keywords: Gene suppression; Huanglongbing; Pest control; RNA interference; RNA silencing
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Year: 2022 PMID： 34495509 DOI： 10.1007/978-1-0716-1633-8_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745