Labrinus van Manen1, Willem A J Birkhoff2, Jeroen Eggermont3, Richelle J M Hoveling4, Philip Nicklin5, Jacobus Burggraaf1,2, Roger Wilson6, J Sven D Mieog1, Dominic J Robinson7, Alexander L Vahrmeijer1, Michelle S Bradbury5,8,9, Jouke Dijkstra3. 1. Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands. 2. Centre for Human Drug Research, Leiden, The Netherlands. 3. Leiden University Medical Center, Division of Image Processing, Department of Radiology, Leiden, The Netherlands. 4. Quest Medical Imaging BV, Middenmeer, The Netherlands. 5. Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY, USA. 6. Department of Anesthesiology, Critical Care Medicine, and Surgery, Memorial Sloan Kettering Cancer Center Research, New York, NY, USA. 7. Erasmus Medical Center, Center for Optical Diagnostics and Therapy, Department of Otorhinolaryngology and Head and Neck Surgery, Rotterdam, The Netherlands. 8. MSK-Cornell Center for Translation of Cancer Nanomedicines, Memorial Sloan Kettering Cancer Center, New York, NY, USA. 9. Molecular Pharmacology Program, Sloan Kettering Institute for Cancer Research, New York, NY, USA.
Abstract
BACKGROUND: Tissue necrosis, a consequence of inadequate tissue oxygenation, is a common post-operative complication. As current surgical assessments are often limited to visual and tactile feedback, additional techniques that can aid in the interrogation of tissue viability are needed to improve patient outcomes. In this bi-institutional pilot study, the performance of a novel snapshot hyperspectral imaging camera to detect superficial cutaneous oxygen saturation (StO2) was evaluated. METHODS: Healthy human volunteers were recruited at two participating centers. Cutaneous StO2 of the forearm was determined by a snapshot hyperspectral camera on two separate study days during occlusion-reperfusion of the brachial artery and after induction of local vasodilation. To calculate the blood StO2 at each pixel in the multispectral image, spectra were selected, and fitting was performed over wavelengths ranging from 470 to 950 nm. RESULTS: Quantitative detection of physiological changes in cutaneous StO2 levels was feasible in all sixteen volunteers. A significant (P<0.001) decrease in cutaneous StO2 levels from 78.3% (SD: 15.3) at baseline to 60.6% (SD: 19.8) at the end of occlusion phase was observed, although StO2 levels returned to baseline after five minutes. Mean cutaneous StO2 values were similar in the same subjects on separate study days (Pearson R2: 0.92 and 0.77, respectively) at both centers. Local vasodilation did not yield significant changes in cutaneous StO2 values. CONCLUSIONS: This pilot study demonstrated the feasibility of a snapshot hyperspectral camera for detecting quantitative physiological changes in cutaneous StO2 in normal human volunteers, and serves as a precursor for further validation in perioperative studies. 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
BACKGROUND: Tissue necrosis, a consequence of inadequate tissue oxygenation, is a common post-operative complication. As current surgical assessments are often limited to visual and tactile feedback, additional techniques that can aid in the interrogation of tissue viability are needed to improve patient outcomes. In this bi-institutional pilot study, the performance of a novel snapshot hyperspectral imaging camera to detect superficial cutaneous oxygen saturation (StO2) was evaluated. METHODS: Healthy human volunteers were recruited at two participating centers. Cutaneous StO2 of the forearm was determined by a snapshot hyperspectral camera on two separate study days during occlusion-reperfusion of the brachial artery and after induction of local vasodilation. To calculate the blood StO2 at each pixel in the multispectral image, spectra were selected, and fitting was performed over wavelengths ranging from 470 to 950 nm. RESULTS: Quantitative detection of physiological changes in cutaneous StO2 levels was feasible in all sixteen volunteers. A significant (P<0.001) decrease in cutaneous StO2 levels from 78.3% (SD: 15.3) at baseline to 60.6% (SD: 19.8) at the end of occlusion phase was observed, although StO2 levels returned to baseline after five minutes. Mean cutaneous StO2 values were similar in the same subjects on separate study days (Pearson R2: 0.92 and 0.77, respectively) at both centers. Local vasodilation did not yield significant changes in cutaneous StO2 values. CONCLUSIONS: This pilot study demonstrated the feasibility of a snapshot hyperspectral camera for detecting quantitative physiological changes in cutaneous StO2 in normal human volunteers, and serves as a precursor for further validation in perioperative studies. 2021 Quantitative Imaging in Medicine and Surgery. All rights reserved.
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