| Literature DB >> 34473331 |
Tannaz Jamialahmadi1,2, Paul C Guest3,4, Amir R Afshari5, Muhammed Majeed6, Amirhossein Sahebkar7,8,9.
Abstract
This chapter presents a protocol for studying the effects of curcumin in a colorectal cell line and a mouse model of colitis-associated colon carcinogenesis. The protocol using the CT26 cell line incorporates cell proliferation, migration, invasion, spheroid formation, cell cycle, polymerase chain reaction (PCR), and western blot analyses. For the mouse model, this involved a macroscopic and histological examination of the colon and assays for oxidative damage markers.Entities:
Keywords: Activity assays; CRC; Colorectal cancer; Curcumin; Histology; Light microscopy; Western blot analysis
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Year: 2022 PMID： 34473331 DOI： 10.1007/978-1-0716-1558-4_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745