Literature DB >> 34473329

Flow Cytometric Analysis of Hyperglycemia-Induced Cell Death Pathways in Cardiomyoblasts.

Ravichandra Shivalingappa Davargaon1, M V V Subramanyam1, S Asha Devi2.   

Abstract

Type-2 diabetes, characterized by hyperglycemia causing various symptoms of metabolic disorders in the heart, kidneys, and brain, has many underlying molecular mechanisms leading to functional insufficiency of these organs. We describe protocols wherein we have optimized conditions for maintenance of hyperglycemic H9c2 cell lines and design to assess the effect of a water-soluble vitamin, Trolox, on the apoptotic pathway. Primarily, the design provides researchers to analyze apoptosis by flow cytometry.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Annexin; Apoptosis; Cardiomyoblast; Flow cytometer; Hyperglycemia; Trolox

Mesh：

Year:  2022        PMID： 34473329     DOI： 10.1007/978-1-0716-1558-4_18

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

1.  Toxic effect of high glucose on cardiomyocytes, H9c2 cells: Induction of oxidative stress and ameliorative effect of trolox.

Authors:  Ravichandra Shivalingappa Davargaon; Asha Devi Sambe; Subramanyam Muthangi V V
Journal:  J Biochem Mol Toxicol       Date:  2018-12-04       Impact factor: 3.642

2.  Metallothionein inhibits doxorubicin-induced mitochondrial cytochrome c release and caspase-3 activation in cardiomyocytes.

Authors:  G W Wang; J B Klein; Y J Kang
Journal:  J Pharmacol Exp Ther       Date:  2001-08       Impact factor: 4.030
  2 in total

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