Zeynep Ergun1. 1. Department of Bioengineering, Faculty of Engineering, Adana Alpaslan Turkes Science and Technology University, Adana, Turkey. zergun@atu.edu.tr.
Abstract
BACKGROUND: Ricinus communis L. (castor bean) is valued for its oil and the performance of oil is closely related to its fatty acid composition. Thus, producing oil in vitro with favored fatty acid profiles is a promising research area and may also offer industrial opportunities. MATERIAL AND METHOD: In line with this, the total amount of oil and the fatty acid composition of the samples, which were endosperm and calli obtained by treatment of various doses of plant growth regulators were determined. RESULTS: Results showed that the type and amount of the plant growth regulator used in the media affect the fatty acid composition. In detail, the biggest change was shown by Indole-3-Acetic Acid (IAA), in general, using the plant growth regulators at 5 mg L-1, instead of 20 mg L-1, was found to have induced larger differentiations. The effect of a natural plant growth regulator (IAA) on fatty acid profiles was bigger than the synthetic ones (NAA, 1-Naphthaleneacetic acid, and 2,4 D, 2,4-Dichlorophenoxyacetic acid). The media containing 5 mg L-1 of NAA, 20 mg L-1 of NAA, 20 mg L-1 of 2,4 D, or 5 mg L-1 of 2,4 D gave similar results.
BACKGROUND: Ricinus communis L. (castor bean) is valued for its oil and the performance of oil is closely related to its fatty acid composition. Thus, producing oil in vitro with favored fatty acid profiles is a promising research area and may also offer industrial opportunities. MATERIAL AND METHOD: In line with this, the total amount of oil and the fatty acid composition of the samples, which were endosperm and calli obtained by treatment of various doses of plant growth regulators were determined. RESULTS: Results showed that the type and amount of the plant growth regulator used in the media affect the fatty acid composition. In detail, the biggest change was shown by Indole-3-Acetic Acid (IAA), in general, using the plant growth regulators at 5 mg L-1, instead of 20 mg L-1, was found to have induced larger differentiations. The effect of a natural plant growth regulator (IAA) on fatty acid profiles was bigger than the synthetic ones (NAA, 1-Naphthaleneacetic acid, and 2,4 D, 2,4-Dichlorophenoxyacetic acid). The media containing 5 mg L-1 of NAA, 20 mg L-1 of NAA, 20 mg L-1 of 2,4 D, or 5 mg L-1 of 2,4 D gave similar results.
Authors: Jefferson da Luz Costa; André Luís Lopes da Silva; Mário César Jucoski Bier; Gilvano Ebling Brondani; André Luiz Gollo; Luiz Alberto Junior Letti; Eduardo Andrea Lemus Erasmo; Carlos Ricardo Soccol Journal: Appl Biochem Biotechnol Date: 2015-04-28 Impact factor: 2.926
Authors: Jeffrey T Foster; Gerard J Allan; Agnes P Chan; Pablo D Rabinowicz; Jacques Ravel; Paul J Jackson; Paul Keim Journal: BMC Plant Biol Date: 2010-01-18 Impact factor: 4.215