Literature DB >> 3446190

Subcellular localization of inositol lipids in blood platelets as deduced from the use of labelled precursors.

G Mauco1, P Dajeans, H Chap, L Douste-Blazy.   

Abstract

1. By rapid fractionation of blood platelet lysates on Percoll density gradients at alkaline pH (9.6), a very pure plasma-membrane fraction was obtained, as well as discrimination between endoplasmic reticulum and lysosomes. 2. Labelling of intact platelets with [32P]Pi followed by subcellular fractionation showed an exclusive localization of all inositol lipids in the plasma membrane. 3. Preincubation of whole platelets with myo-[3H]inositol in a buffer containing 1 mM-MnCl2 allowed incorporation of the label into PtdIns (phosphatidylinositol) of both plasma and endoplasmic-reticulum membrane, whereas [3H]PtdIns4P (phosphatidylinositol 4-phosphate) and [3H]PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) were exclusively found on the plasma membrane. 4. It is concluded that PtdIns4P and PtdIns(4,5)P2 are exclusively localized in the plasma membrane, whereas PtdIns is present in both plasma and endoplasmic-reticulum membranes. This could provide an explanation for previously reported data on hormone-sensitive and -insensitive inositol lipid pools.

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Year:  1987        PMID： 3446190      PMCID： PMC1148060          DOI： 10.1042/bj2440757

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  51 in total

Review 1.  Inositol phospholipids and cell surface receptor function.

Authors:  R H Michell
Journal:  Biochim Biophys Acta       Date:  1975-03-25

2.  Synthesis of molecular classes of cytidine diphosphate diglyceride by rat liver in vivo and in vitro.

Authors:  W Thompson; G MacDonald
Journal:  Can J Biochem       Date:  1977-11

3.  Guanine nucleotides decrease the free [Ca2+] required for secretion of serotonin from permeabilized blood platelets. Evidence of a role for a GTP-binding protein in platelet activation.

Authors:  R J Haslam; M M Davidson
Journal:  FEBS Lett       Date:  1984-08-20       Impact factor: 4.124

4.  Thin-layer chromatography of polyphosphoinositides from platelet extracts: interference by an unknown phospholipid.

Authors:  O B Tysnes; G M Aarbakke; A J Verhoeven; H Holmsen
Journal:  Thromb Res       Date:  1985-11-01       Impact factor: 3.944

5.  The relationship of hormone-sensitive and hormone-insensitive phosphatidylinositol to phosphatidylinositol 4,5-bisphosphate in the WRK-1 cell.

Authors:  K Koréh; M E Monaco
Journal:  J Biol Chem       Date:  1986-01-05       Impact factor: 5.157

6.  Stimulation of phosphatidic acid production in platelets precedes the formation of arachidonate and parallels the release of serotonin.

Authors:  E G Lapetina; P Cuatrecasas
Journal:  Biochim Biophys Acta       Date:  1979-05-25

7.  Secretagogue-responsive and -unresponsive pools of phosphatidylinositol in pancreatic islets.

Authors:  R S Rana; A Kowluru; M J MacDonald
Journal:  Arch Biochem Biophys       Date:  1986-03       Impact factor: 4.013

8.  Biochemical characterization of plasma membranes and intracellular membranes isolated from human platelets using Percoll gradients.

Authors:  J Fauvel; H Chap; V Roques; S Levy-Toledano; L Douste-Blazy
Journal:  Biochim Biophys Acta       Date:  1986-03-27

9.  Human platelets contain phospholipase C that hydrolyzes polyphosphoinositides.

Authors:  S E Rittenhouse
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

10.  Phosphorylation of rat liver nuclear envelopes. I. Characterization of in vitro protein phosphorylation.

Authors:  C D Smith; W W Wells
Journal:  J Biol Chem       Date:  1983-08-10       Impact factor: 5.157
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  5 in total

1.  Reversal of the Ca2+ pump of blood platelets.

Authors:  J C Benech; H Wolosker; L de Meis
Journal:  Biochem J       Date:  1995-02-15       Impact factor: 3.857

2.  Thyrotropin-releasing hormone receptor occupancy determines the fraction of the responsive pool of inositol lipids hydrolysed in rat pituitary tumour cells.

Authors:  A B Cubitt; E Geras-Raaka; M C Gershengorn
Journal:  Biochem J       Date:  1990-10-15       Impact factor: 3.857

3.  The novel inositol lipid phosphatidylinositol 3,4-bisphosphate is produced by human blood platelets upon thrombin stimulation.

Authors:  C Sultan; M Breton; G Mauco; P Grondin; M Plantavid; H Chap
Journal:  Biochem J       Date:  1990-08-01       Impact factor: 3.857

4.  Phospholipases of mineralization competent cells and matrix vesicles: roles in physiological and pathological mineralizations.

Authors:  Saida Mebarek; Abdelkarim Abousalham; David Magne; Le Duy Do; Joanna Bandorowicz-Pikula; Slawomir Pikula; René Buchet
Journal:  Int J Mol Sci       Date:  2013-03-01       Impact factor: 5.923

5.  Association of profilin with filament-free regions of human leukocyte and platelet membranes and reversible membrane binding during platelet activation.

Authors:  J H Hartwig; K A Chambers; K L Hopcia; D J Kwiatkowski
Journal:  J Cell Biol       Date:  1989-10       Impact factor: 10.539
  5 in total

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