Literature DB >> 34458502

3D Stroma Invasion Assay.

Yvette May Coulson-Thomas1, Vivien Jane Coulson-Thomas2.   

Abstract

We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of colorectal cancer cells through the surrounding stroma. This protocol has been previously published (Coulson- Thomas et al., 2011 ) and is described here in more detail.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  3D culture; Cancer cell invasion; Cancer cells; Desmoplastic reaction; Fibroblasts; Stromagenic system

Year:  2017        PMID： 34458502      PMCID： PMC8376574          DOI： 10.21769/BioProtoc.2195

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  Enterolobium contortisiliquum trypsin inhibitor (EcTI), a plant proteinase inhibitor, decreases in vitro cell adhesion and invasion by inhibition of Src protein-focal adhesion kinase (FAK) signaling pathways.

Authors:  Cláudia Alessandra Andrade de Paula; Vivien Jane Coulson-Thomas; Joana Gasperazzo Ferreira; Paloma Korehisa Maza; Erika Suzuki; Adriana Miti Nakahata; Helena Bonciani Nader; Misako Uemura Sampaio; Maria Luiza V Oliva
Journal:  J Biol Chem       Date:  2011-10-28       Impact factor: 5.157

2.  Colorectal cancer desmoplastic reaction up-regulates collagen synthesis and restricts cancer cell invasion.

Authors:  Vivien J Coulson-Thomas; Yvette M Coulson-Thomas; Tarsis F Gesteira; Cláudia A A de Paula; Ana M Mader; Jaques Waisberg; Maria A Pinhal; Andreas Friedl; Leny Toma; Helena B Nader
Journal:  Cell Tissue Res       Date:  2011-10-11       Impact factor: 5.249
  2 in total

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