Literature DB >> 34458475

Transient Transfection-based Fusion Assay for Viral Proteins.

Melina Vallbracht1, Christina Schröter1, Barbara G Klupp1, Thomas C Mettenleiter1.   

Abstract

Membrane fusion is vital for entry of enveloped viruses into host cells as well as for direct viral cell-to-cell spread. To understand the fusion mechanism in more detail, we use an infection free system whereby fusion can be induced by a minimal set of the alphaherpesvirus pseudorabies virus (PrV) glycoproteins gB, gH and gL. Here, we describe an optimized protocol of a transient transfection based fusion assay to quantify cell-cell fusion induced by the PrV glycoproteins.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Glycoproteins gB; Herpesvirus; Membrane fusion; Pseudorabies virus; Transfection; Virus entry; gH/gL

Year:  2017        PMID： 34458475      PMCID： PMC8376598          DOI： 10.21769/BioProtoc.2162

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  Analysis of fusion using a virus-free cell fusion assay.

Authors:  Marisa P McShane; Richard Longnecker
Journal:  Methods Mol Biol       Date:  2005

2.  Mutations in Pseudorabies Virus Glycoproteins gB, gD, and gH Functionally Compensate for the Absence of gL.

Authors:  Christina Schröter; Melina Vallbracht; Jan Altenschmidt; Sabrina Kargoll; Walter Fuchs; Barbara G Klupp; Thomas C Mettenleiter
Journal:  J Virol       Date:  2015-12-09       Impact factor: 5.103

3.  Characterization of cell-cell fusion mediated by herpes simplex virus 2 glycoproteins gB, gD, gH and gL in transfected cells.

Authors:  Martin I Muggeridge
Journal:  J Gen Virol       Date:  2000-08       Impact factor: 3.891

4.  Adaptability in herpesviruses: glycoprotein D-independent infectivity of pseudorabies virus.

Authors:  J Schmidt; B G Klupp; A Karger; T C Mettenleiter
Journal:  J Virol       Date:  1997-01       Impact factor: 5.103

Review 5.  Viral membrane fusion.

Authors:  Stephen C Harrison
Journal:  Virology       Date:  2015-04-10       Impact factor: 3.616

6.  Glycoproteins gB, gD, and gHgL of herpes simplex virus type 1 are necessary and sufficient to mediate membrane fusion in a Cos cell transfection system.

Authors:  A Turner; B Bruun; T Minson; H Browne
Journal:  J Virol       Date:  1998-01       Impact factor: 5.103
  6 in total
  1 in total

1.  In Vitro Viral Evolution Identifies a Critical Residue in the Alphaherpesvirus Fusion Glycoprotein B Ectodomain That Controls gH/gL-Independent Entry.

Authors:  Melina Vallbracht; Henriette Lötzsch; Barbara G Klupp; Walter Fuchs; Benjamin Vollmer; Kay Grünewald; Marija Backovic; Felix A Rey; Thomas C Mettenleiter
Journal:  mBio       Date:  2021-05-04       Impact factor: 7.867
  1 in total

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